吉林大学学报（医学版）2019，Vol.45Issue(1)：69-72,4.DOI:10.13481/j.1671-587x.20190113
白藜芦醇对肝癌HepG2细胞中脂肪合成的抑制作用及其机制
Inhibitory effect of resveratrol on fat synthesis in liver cancer HepG2 cells and its mechanism
摘要
Abstract
Objective:To investigate the effect of resveratrol (Res) on the fat synthesis in the liver cancer HepG2cells, and to elucidate its possible mechanism.Methods:The HepG2cells were cultured in vitro and divided into Res group (treated with 40μmol·L-1 DMSO-diluted Res for 24h) and control group (treated with the same concentration of DMSO for 24h) .The cell supernatant was collected, and the levels of triglyceride (TG) and total cholesterol (TC) in the cells in various groups were measured by ELISA.The mRNA and protein expression levels of lipase synthase acetyl-CoA carboxylase (ACC1) , fatty acid synthetase (FASN) and stearoyl-CoA desaturase (SCD1) in the cells in various groups were detected by qRT-PCR and Western blotting method.The levels of O-linked N-acetylglucosamine (O-GlcNAc) glycosylation in the cells in various groups were detected by Western blotting method.Results:Compared with control group, the levels of TG and TC in the cells in Res group were decreased, but the difference was not statistically significant (t1=1.886, P＞0.05;t2=2.457, P＞0.05) .Compared with control group, the levels of expressions of ACC1, FASN and SCD1mRNA and proteins in the cells in Res group were significantly decreased (P＜0.05or P＜0.01) ;the O-GlcNAc glycosylation level in the cells in Res group was significantly decreased (t=2.87, P＜0.05) .Conclusion:Res has the effect of inhibiting the fat synthesis in the liver cancer HepG2 cells.Its mechanism may be related to the reduction of cellular O-GlcNAc glycosylation level and the reduction of the expression of FASN.关键词
白藜芦醇/非酒精性脂肪肝/肝癌HepG2细胞/脂肪酸合成酶/氧链接N乙酰葡糖胺糖基化Key words
resveratrol/non-alcoholic fatty liver disease/HepG2 cells/fatty acid synthetase/O-GlcNAc glycosylation分类
医药卫生引用本文复制引用
苗向霞,郭蕊,张璎,卫银银,罗正奇,闵亚丽,刘凯歌..白藜芦醇对肝癌HepG2细胞中脂肪合成的抑制作用及其机制[J].吉林大学学报（医学版）,2019,45(1):69-72,4.基金项目
陕西省教育厅科研项目资助课题(11JK0708) （11JK0708）
陕西省科技厅科研项目资助课题(2016SF-327) （2016SF-327）
