草业学报2019，Vol.28Issue(1)：86-94,9.DOI:10.11686/cyxb2018095
虉草适应湿地环境的解剖结构和组织化学特征研究
Anatomy and histochemistry features of Phalaris arundinacea adapted to wetland environments
摘要
Abstract
Reed canarygrass (Phalaris arundinacea) is a perennial herb adapted to wetl and environments.The current work explores the anatomy and histochemistry of the adventitious roots, stems (rhizomes and culms), leaves and the permeability of apoplastic barriers of reed canarygrass.The anatomy and histochemistry of P.arundinacea were studied using an optical microscope and fluorescent microscope.Sections were stained with Sudan red 7B (SR7B) for suberin lamellae, with berberine hemisulfate-aniline blue (BAB) for casparian b ands and lignified cell walls, phloroglucinol-HCl (Pg) for lignin and berberine hemisulfate-potassium thiocyanate for apoplastic permeability.Concentrated sulfuric acid digestion (AD) was used to detect the wavy casparian b ands in young rhizomes and culms.The adventitious roots had a primary structure comprising epidermis, exodermis, cortex, endodermis and vascular cylinder with sclerenchyma;the stems comprised a cuticle, epidermis, peripheral mechanical ring, exodermis, cortex, endodermis, sclerenchyma ring with vascular bundles and pith cavity.The apoplastic barriers consisted of adventitious roots with a suberized and lignified endodermis and an exodermis, stems possessed a cuticle, suberized and lignified peripheral mechanical ring and a sclerenchyma ring, and rhizomes with endodermis and exodermis, culms only with exodermis.The air space consisted of pith cavities and cortical cavities in the stems, and aerenchyma in roots and leaves.This research revealed that reed canarygrass has the key constitutive, structural and histochemical features in roots and stems that allow it to grow in wetl ands, be protected against oxygen leakage from aerenchyma, with-st and seasonal flooding and consequently, widely distributed.The anatomical structures and histochemical features of these perennial, rhizomatous grasses enhance our awareness of the biology of this species in native Asian and North American invaded habitats to select species for the restoration of degraded wetl and of the Jianghan Floodplain and Three Gorges Reservoir Region of the Yangtze River.关键词
虉草/解剖结构/质外体屏障结构/组织化学Key words
Phalaris arundinacea/anatomy/apoplastic barriers/histochemistry引用本文复制引用
王晓娥,张梵,张霞,周存宇,杨朝东..虉草适应湿地环境的解剖结构和组织化学特征研究[J].草业学报,2019,28(1):86-94,9.基金项目
中国科学院武汉植物园水生植物与流域生态重点实验室开放基金(2017)资助 （2017）
