草业学报2019，Vol.28Issue(1)：120-127,8.DOI:10.11686/cyxb2018099
利用CRISPR/Cas9技术敲除拟南芥转录因子MYB40的两种可变剪接体
Knock out of two splice variants of MYB40 using the gene-editing technique CRISPR/Cas9
摘要
Abstract
MYB transcription factors play important roles in various abiotic stress signaling pathways.In a previous study, we found that ZxMYB315 from Zygophyllum xanthoxylumis involved in plant responses to salt and drought stresses.In addition, the ortholog of ZxMYB315 in Arabidopsis, MYB40, has two splice variants, MYB40.1 and MYB40.2.Expression levels of both MYB40.1 and MYB40.2 are affected by salt treatment.However, the functions of these changes in response to abiotic stress are still unclear.In this study, we further analyzed the expression patterns of MYB40.1 and MYB40.2 under salt treatment.In particular, two heterozygous mutations for MYB40.1 and three heterozygous mutations for both MYB40.1 and MYB40.2 were generated using the gene-editing technique CRISPR/Cas9.These mutants now provide a foundation for studying functions and molecular mechanisms of MYB40 in various abiotic stress signaling pathways.关键词
转录因子/MYB40.1/MYB40.2/抗逆/CRISPR/Cas9技术Key words
transcription factor/MYB40.1/MYB40.2/abiotic stress tolerance/CRISPR/Cas9引用本文复制引用
李孟湛,尹红菊,李丁丁,刘亚琪,王锁民..利用CRISPR/Cas9技术敲除拟南芥转录因子MYB40的两种可变剪接体[J].草业学报,2019,28(1):120-127,8.基金项目
国家自然科学基金青年基金项目(31601992)资助 （31601992）
