草业学报2019，Vol.28Issue(1)：150-161,12.DOI:10.11686/cyxb2018303
基于转录组测序分析象草木质素合成的研究
De novo transcriptomic analysis for lignin synthesis in Cenchrus purpureus using RNA-seq
摘要
Abstract
Elephant grass (Cenchrus purpureus) is an excellent forage crop and a promising lignocelluloses energy crop;its stem characteristics are very important for forage and feedstock quality.In order to underst and the gene expression profile of elephant grass stem in the absence of a reference genome, we performed de novo transcriptome sequencing and compared the transcriptome of stems with a high lignin content plant (HLCP) eg7 and a low lignin content plant (LLCP) eg87 in elephant grass using Illumina HiSeq 2000 platform.A total of 169630902 high qualities reads were obtained, which were then assembled into 87641 unigenes with an average length of 580nt.All of the 87641 unigenes were compared against the non-redundant protein database (Nr), non-redundant nucleotide database (Nt), swisssprot protein sequence database (Swiss-Prot), gene ontology (GO), cluster of orthologous groups (COG) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases for functional annotation;a total of 62557 unigenes were annotated.Most homologous sequences were found between elephant grass with sorghum.33323unigenes showed significant differences in expression between eg7 and eg87 (control), 9704 (29.1％ ) genes were up-regulated and 23619 (70.9％ ) were down-regulated.GO and KEGG pathway analysis revealed that the genes related to photosynthesis-antenna proteins, photosynthesis, betalain biosynthesis, phenylpropanoid biosynthesis are genes of interest.9monolignol biosynthesis-related genes and 8 ClassⅢperoxidase genes were selected as c andidate genes.qRT-PCR analysis showed that the expression of 9 selected unigenes were consistent with the transcriptome data.Our study presented comprehensive transcriptomic data and gene function analysis of C.purpureus stem, providing a valuable resource for future studies of plant breeding in the genus Pennisetum and comparative genome analysis for C4 grasses.关键词
象草/高通量测序/转录组/木质素Key words
Cenchrus purpureus/high-throughput sequencing/transcriptome/lignin引用本文复制引用
吴娟子,钱晨,刘智微,潘玉梅,钟小仙..基于转录组测序分析象草木质素合成的研究[J].草业学报,2019,28(1):150-161,12.基金项目
国家自然科学基金项目(31302025),江苏省农业科技自主创新资金(CX(17)3051)和江苏省农业科学院探索性颠覆性创新计划项目[ZX(17)2017]资助 （31302025）
