草业学报2019，Vol.28Issue(1)：162-169,8.DOI:10.11686/cyxb2018091
翻压接种根瘤菌的紫花苕子对植烟土壤肥力的影响
Effects of a Vicia villosa green-manure crop inoculated rhizobium during winter fallow, on soil fertility factors and fertilizer needs of a summer tobacco crop
摘要
Abstract
In order to investigate the effect on crop yield and soil fertility of Vicia villosaas a green manure crop in tobacco growing, a field experiment comprising three treatments was set up:winter fallow without a green manure crop (CK), a winter green manure crop of V.villosa not inoculated with rhizobium (V), and a winter green manure crop of rhizobium-inoculated V.villosa (VR).Plants of V.villosa were harvested for measurement at the beginning of flowering (187 days after planting) to determine their yield and nutrient levels, and then ploughed under, with tobacco planting following 45days later.For tobacco planting on V and VR plots, fertilizer was applied at 80％ of the rates recommended by the Guangyuan Tobacco Company for the CK treatment.Before planting tobacco and after harvest, soil samples were collected and physicochemical traits, enzyme activity and microbial populations were determined.Key results were:1) Rhizobium inoculation enhanced vigor of the V.villosa green manure crop.Plant biomass and nutrient content, fresh grass yield, hay yield, root yield, nodule numbers, and plant height were all increased by 1.3％ to 50.7％;total N, total P, and total K were increased by 1.8％ to 5.0％;N, P, and K accumulation increased by 14.3％ to 21.5％.2) In the soil tests taken before planting tobacco, the VR treatment had increased soil nutrient content and microbial populations, compared with the CK treatment.Specifically, total N, organic matter, and available N, P, and K increased by 2.5％ to 126.6％.Meanwhile, the activities of sucrase, catalase, phosphatase and urease were increased by 14.6％ to 82.5％, while populations of bacteria, actinomycetes and fungi were increased by 85.3％ to 236.9％.In the soil tests taken after harvesting tobacco, it was found that the VR treatment increased soil nutrient content and microbial populations, even with the 20％ reduction in chemical fertilizer application.Total N, organic matter, and available N, P, and K were increased by 12.3％ to 64.1％, the activities of sucrose, catalase, phosphatase and urease increased by 5.4％ to 67.0％, compared to CK, while populations of bacteria, actinomycetes and fungi were increased by 65.1％ to 473.3％.This finding that use of rhizobium-inoculatedd V.villosa (i.e.the VR treatment) can improve soil fertility status even with a 20％ reduction in inorganic fertilise application, is of great significance to development of more sustainable tobacco leaf production practices.关键词
紫花苕子/根瘤菌/土壤酶活性/微生物数量Key words
Vicia villosa/rhizobium/soil enzyme activities/number of microorganisms引用本文复制引用
刘明,陈远学,陈强,彭丹,喻晓,杨军伟,徐开未..翻压接种根瘤菌的紫花苕子对植烟土壤肥力的影响[J].草业学报,2019,28(1):162-169,8.基金项目
中国烟草总公司四川省公司科技专项项目"植烟土壤质量提升关键技术研究与集成应用"(SCYC201504)资助 （SCYC201504）
