草业学报2019，Vol.28Issue(2)：102-111,10.DOI:10.11686/cyxb2018131
耐重金属苜蓿中华根瘤菌的筛选及其与能源植物联合富集铜的特性
Isolation of heavy metal-tolerant Sinorhizobium meliloti and the effect on copper uptake of alfalfa, perennial ryegrass and Sorghum bicolor plants grown on copper-contaminated soil
摘要
Abstract
This research was aimed at improvement of the adaptability and performance of plants used for phytoremediation in marginal lands subject to heavy metal contamination, The use of rhizobia to increase biomass productivity is an exciting new development in the phytoremediation of contaminated sites.In the present study, a heavy-metal-tolerant rhizobia strain was isolated from nodules of alfalfa (Medicago sativa) plants grown in copper (Cu) mine tailings in Nanjing.A greenhouse experiment was conducted to evaluate the potential role of this rhizobium strain in encouraging plant growth, Cu uptake and soil remediation by three plant species:M.sativa, Lolium perenne and Sorghum bicolor.The strain of Sinorhizobium meliloti (designated D10) was isolated from root nodules of alfalfa, and was Cu-Pb-Cd-tolerant.Symbiotic association was successfully established between S.meliloti D10 and M.sativain the current research.It was found that the biomass production of the three species ranked in order:S.bicolor＞L.perenne＞M.sativa.Inoculation of S.meliloti D10 significantly increased the biomass of M.sativaand S.bicolor, with a 28.6％-78.1％ greater biomass for inoculated vs.uninoculated plants.Among the tested plants, S.bicoloraccumulated the greatest amounts of Cu.Further, inoculation of S.meliloti D10 resulted in a significant increase in Cu uptake in S.bicolorand M.sativaof50.4％-111.8％ of the uninoculated control.The beneficial impacts of S.meliloti D10 colonization on plant growth and Cu uptake of the three tested species was associated with both improved water-soluble sugar content and increased urease activity.The experiment provided evidence of the potential for use of these plant species in combination with S.meliloti D10 for ecological remediation of metalliferous mine tailings.关键词
根瘤菌/能源植物/铜/边际土壤/土壤修复Key words
rhizobia/energy plant/copper/marginal lands/soil remediation引用本文复制引用
严警,夏丽,盛下放,何琳燕..耐重金属苜蓿中华根瘤菌的筛选及其与能源植物联合富集铜的特性[J].草业学报,2019,28(2):102-111,10.基金项目
国家自然科学基金(No.41471273) （No.41471273）
江苏省社会发展项目(No.BE2013710)资助 （No.BE2013710）
