长春中医药大学学报2019，Vol.35Issue(1)：1-5,5.DOI:10.13463/j.cnki.cczyy.2019.01.001
双特异性溶瘤腺病毒通过调节活性氧引起人肺癌细胞凋亡
Oncolytic Adenovirus Induced Human Lung Cancer Cell Apoptosis via Regulating Reactive Oxygen
摘要
Abstract
Objective To investigate the relationship between apoptosis and the level of reactive oxygen in ATV-infected A549 cells.Methods The dual cancer-specific oncolytic adenovirus ATV was constructed in our laboratory.The suppression effect of ATV-infected A549 cells was detected by CCK-8 cell proliferation assay.The apoptosis of ATV-infected A549 cells was analyzed by Hoechst staining and Annexin V-FITC/PI flow cytometer detection.The level of reactive oxygen in ATV-infected A549 cells was evaluated by human reactive oxygen Elisa kit.Results The results of CCK-8 cell proliferation assay showed that A549 cells were suppressed significantly by 10 MOI or 50 MOI ATV at 48 h and 72 h.The results of hoechst staining were showed that typical characteristics of apoptosis and typical nuclear fragmentation in ATV-infected A549 cells could be observed.The result of Annexin V-FITC/PI flow detection was showed that apoptosis of A549 cells was induced by ATV at 12 h, 24 h, 48 h and 72 h, and the apoptosis rate increased with time.The result of cytoplasm reactive oxygen detection was showed that the level of reactive oxygen in ATV-infected A549 cells increases with time at 12 h, 24 h, 48 h and 72 h, which has a significant time effect.Conclusion ATV has suppression effect on A549 cells, and this effect has a clear time-dependent and dose-dependent relationship.The apoptosis of A549 cells can be induced by ATV via causing the increase of reactive oxygen level in A549 cell.关键词
溶瘤腺病毒/活性氧/A549/凋亡Key words
oncolytic adenovirus/reactive oxygen/A549/apoptosis分类
医药卫生引用本文复制引用
白冰,张冬娜,蒋本春,房金波,马艺珍,朱羿龙,朱光泽,李霄,金宁一..双特异性溶瘤腺病毒通过调节活性氧引起人肺癌细胞凋亡[J].长春中医药大学学报,2019,35(1):1-5,5.基金项目
国家新药创制科技重大专项项目(2018ZX09301053-004-001). （2018ZX09301053-004-001）
