茶叶学报2018，Vol.59Issue(4)：181-185,5.
茶树细胞色素P450基因CYP710A1的克隆及差异表达特征分析
Cloning and Expression of CYP710A1 Gene of Cytochrome P450 in Camellia Sinensis
摘要
Abstract
Cytochrome P450 plays an important role in disease-resistance and insect infestation of tea plants.This study cloned the full cDNA sequence of cytochrome P450 CYP710 A1 gene of Camellia sinensis by RT-PCR and 5'/3'RACE based on the NCBI transcriptome database.The amino acid sequence, physicochemical properties, functional domain and phylogenetic tree of CYP710 A1 were determined using bioinformatics software, while the mRNA of the leaves at different positions on a plant compared applying qRT-PCR data.The results showed that the length of a complete cDNA of CYP710 A1 was 1, 911 bp encoded 502 amino acids with a molecular weight of57.06 kDa and the theoretical isoelectric point at 7.64.The gene consisted of a P450 Pfam domain with a transmembrane structure.A phylogenetic tree analysis showed that CYP710 A1 had a genetic relationship closest with Capsicum baccatumand farthest with Macleaya cordata.The qRT-PCR results indicated that the third leaves on a branch tip had the highest expression of CYP710 A1 gene, and the buds the lowest-with a 38.17-fold difference between them.关键词
茶树/CYP710A1基因/克隆/叶片/表达含量Key words
Camellia sinensis/CYP710A1 gene/clone/leaves/expression levels分类
农业科技引用本文复制引用
单睿阳,陈常颂,钟秋生,林郑和,陈志辉,游小妹..茶树细胞色素P450基因CYP710A1的克隆及差异表达特征分析[J].茶叶学报,2018,59(4):181-185,5.基金项目
国家茶叶产业技术体系(CARS-19) （CARS-19）
福建省农业科学院茶叶科技创新团队建设项目(2016cytd) （2016cytd）
福建省农业科学院"十三五"青年创新团队项目(STIT2017-3-12) （STIT2017-3-12）
福建省财政厅项目(20151297) （20151297）
福建省属公益类科研院所基本科研专项(2016R1011-3). （2016R1011-3）
