动物营养学报2019，Vol.31Issue(1)：131-139,9.DOI:10.3969/j.issn.1006-267x.2019.01.018
L-精氨酸对冷应激仔猪生长性能、免疫功能及肝脏、肾脏中肿瘤坏死因子-α、干扰素-γ基因表达量的影响
Effects of L-Arginine on Growth Performance, Immune Function and Genes Expression Levels of Tumor Necrosis Factor-α and Interferon-γ in Liver and Kidney of Cold-Stressed Piglets
摘要
Abstract
The aim of this experiment was to investigate the effects of L-arginine (L-Arg) on growth performance, immune function and gene expression levels of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in the liver and kidney of cold stressed piglets.The experiment was designed with two factors with repeated complete cross factorial test.According to body weight and sexual distinction, a total of 72 piglets with28 days of weaning and similar weight [ (7.48±0.47) kg]were selected and divided into 6 groups with 6 repetitions per group and 2 piglets per repetition, and cross treated with two temperatures (normal temperature 23 to 27 ℃, low temperature 14 to 18 ℃) and three levels of L-Arg supplementation (0, 0.8％ and 1.2％) .The experiment lasted for 21 d.The results showed as follows:1) temperature (P=0.006) and L-Arg (P=0.027) had significant influence on the average daily gain of piglets, and their interaction could significantly increase the average daily gain of piglets (P=0.047) .The temperature, L-Arg and their interaction had no significant influence on the average daily feed intake of piglets (P> 0.05) .Temperature (P=0.031) and L-Arg (P=0.037) had significant effects on the ratio of feed to gain, and their interaction had no significant effect on the ratio of feed to gain (P=0.269) .2) Temperature and L-Arg had significant influence on the weight of thymus and spleen of piglets (P<0.05) , and their interaction had no significant effect on thymus weight (P=0.352) , while significantly affected the weight of spleen (P=0.026) .3) The interaction of temperature and L-Arg had significant effects on serum immunoglobulin (Ig) A (P=0.049) , IgM (P=0.021) and IgG (P=0.015) contents.4) The interaction of temperature and L-Arg had significant effects on the gene expression level of IFN-γ in the liver (P=0.018) , and had no significant effect on the gene expression levels of TNF-α and IFN-γ in the kidney (P>0.05) .The results show that adding L-Arg to the diet can improve the growth performance and immune function of the cold-stressed piglets.关键词
L-Arg/冷应激/生长性能/免疫功能/基因表达量Key words
L-Arg/cold stress/growth performance/immune function/gene expression level分类
农业科技引用本文复制引用
黄思琪,曲红焱,黄大鹏,耿忠诚..L-精氨酸对冷应激仔猪生长性能、免疫功能及肝脏、肾脏中肿瘤坏死因子-α、干扰素-γ基因表达量的影响[J].动物营养学报,2019,31(1):131-139,9.基金项目
黑龙江省农垦总局科技攻关项目 (HNK135-04-06, HNKXIV-08-03-01) （HNK135-04-06, HNKXIV-08-03-01）
