动物营养学报2019，Vol.31Issue(1)：258-265,8.DOI:10.3969/j.issn.1006-267x.2019.01.032
大麻素受体1拮抗剂利莫那班对肉仔鸡下丘脑食欲调控因子和腺苷酸激活蛋白激酶基因表达的影响
Effects of Cannabinoid Receptor 1 Antagonist Rimonabant on Gene Expression of Hypothalamic Appetite Regulation Factor and Adenosine Monophosphate-Activated Protein Kinase of Broilers
摘要
Abstract
This study aimed to investigate the effects of cannabinoid receptor 1 (CB1) antagonist rimonabant (SR141716) on gene expression of hypothalamic appetite regulation factor and adenosine monophosphate-activated protein kinase (AMPK) of broilers.Twenty healthy one-day-old Arbor Acres (AA) broilers with similar weight were randomly allocated into 2 groups at 7 days of age, and a guide cannula were implanted stereotaxically into the third ventricle, chicks were allowed 3 days of recovery.At 08:00 at 10 days of age, birds in the experimental group were ventricle injected 1 μg SR141716, while other birds in the control group injected the same amount of dimethyl sulfoxide (DMSO) at the same time.The feed intake of each chick in the 2 hours after injection was counted, then the blood and hypothalamic sample was taken, the activities of alanine aminotransferase, aspartate aminotransferase and contents of glucose, uric acid, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol in serum were determined.The gene expression of AMPKα1, AMPKα2, pro-opiomelanocortin-α (POMC) , neuropeptide Y (NPY) , agouti-related protein (AgRP) and cocaine-and amphetamine-regulated transcript (CART) in hypothalamus were detected by real time PCR method.The results showed as follows:1) compared with the control group, the feed intake of broilers was significantly decreased in the 2 hours after ventricle injected SR141716 (P<0.05) .2) Compared with the control group, the serum low density lipoprotein cholesterol content of broilers was significantly increased after ventricle injected SR141716 (P<0.05) , but there was no significant effect on other serum biochemical parameters (P> 0.05) .3) Compared with the control group, the gene expression of AMPKα1, AMPKα2, POMC and AgRP in hypothalamus of broilers were significantly decreased after ventricle injected SR141716 (P<0.05) , while the gene expression of CART in hypothalamus was significantly increased (P<0.05) , but there was no significant effect on the gene expression of NPY in hypothalamus (P>0.05) .In conclusion, ventricle injected CB1 antagonist SR141716 can reduce the feed intake of broilers and affect the gene expression of AMPK and its downstream appetite regulator POMC in central nervous.The central nervous is involved in the appetite regulation of broilers by inhibiting the stimulate neuropeptide AgRP and anorexigenic neuropeptides CART.关键词
肉仔鸡/SR141716/大麻素受体/腺苷酸激活蛋白激酶/食欲Key words
broilers/SR141716/cannabinoid receptor/AMPK/appetite分类
农业科技引用本文复制引用
周华金,孙晓蕾,钟光,张辉,王继光,郝洋洋,宋志刚..大麻素受体1拮抗剂利莫那班对肉仔鸡下丘脑食欲调控因子和腺苷酸激活蛋白激酶基因表达的影响[J].动物营养学报,2019,31(1):258-265,8.基金项目
国家自然科学基金项目 (31472115) （31472115）
山东省家禽产业创新团队项目 (STAIT-011-08) （STAIT-011-08）
