动物营养学报2019，Vol.31Issue(1)：314-323,10.DOI:10.3969/j.issn.1006-267x.2019.01.038
采用Illumina MiSeq测序技术分析葡萄籽原花青素对奶牛体外瘤胃发酵产甲烷菌区系的影响
Effects of Grape Seed Procyanidine on Methanogens Flora of in Vitro Rumen Fermentation of Dairy Cows Using Illumina MiSeq Sequencing Technology
摘要
Abstract
The present study was designed to explore effects of grape seed procyanidine on Methanogens flora of in vitro rumen fermentation of dairy cows using Illumina MiSeq sequencing technology.The trial was divided into 6 groups.Total mixed ration with the concentrate to forage ratio at 40:60 was used as the fermentation substrate and added grape seed procyanidin at levels of 0 (control) , 0.1, 0.2, 0.3, 0.4 and 0.5 g/kg, respectively.Total DNA was extracted from fermentation liquor after 24 h fermentation, and sequenced by Illumina MiSeq PE300 high-throughput sequencing platform.The results showed as follows:1) compared with the control group, the supplementation of grape seed procyanidins at 0.1, 0.2 g/kg significantly decreased the operational taxonomic units (OTUs) , while the supplementation of grape seed procyanidins at 0.3, 0.4 and 0.5 g/kg increased the OTUs, but did not have significant difference (P>0.05) ;the supplementation of grape seed procyanidins did not significantly affect Chao1 index and Shannon index (P>0.05) , namely did not significantly affect the diversity of Methanogens.2) At phyla level, compared with the control group, the supplementation of grape seed procyanidine at 0.3, 0.4 and 0.5 g/kg significantly decreased the abundance of Euryarchaeota (P<0.05) .At family level, compared with the control group, the abundance of Methanobacteriaceae had a decreased trend with the supplementation of grape seed procyanidine increasing, and when the supplementation was at 0.4 g/kg, the abundance of it significantly decreased (P<0.05) ;the abundance of Methanomassiliicoccaceae increased with the supplementation of grape seed procyanidine increasing, and when the supplementation was 0.3 and 0.4 g/kg, the abundance of it significantly increased (P<0.05) .At genus level, compared with the control group, the abundance of Methanobrevibacter decreased with the supplementation of grape seed procyanidine increasing, and when the supplementation was 0.4 g/kg, the abundance of it significantly decreased (P<0.05) , when the supplementation was 0.5 g/kg, the abundance of it had an increasing trend, but was lower than that in control group;the abundance of Methanobacterium decreased with the supplementation of grape seed procyanidine increasing, and when the supplementation was 0.4 and 0.5 g/kg, the abundance of it significantly decreased (P<0.05) ;the abundance of Candidatus_Methanoplasma had an increasing trend with the supplementation of grape seed procyanidine increasing, and when supplementation was 0.3 and 0.4 g/kg, the abundance of it significantly increased (P<0.05) ;the abundance of Methanosphaera decreased with the supplementation of grape seed procyanidine increasing, and when the supplementation was 0.3, 0.4 and 0.5 g/kg, the abundance of it significantly decreased (P< 0.05) .Therefore, the supplementation of grape seed procyanidine has significant effects on Methanogens at phyla, family and genus levels.关键词
葡萄籽原花青素/奶牛/产甲烷菌/Illumina MiSeq测序技术Key words
grape seed procyanidins/dairy cows/Methanogens/Illumina MiSeq sequencing technology分类
农业科技引用本文复制引用
童津津,张华,孙铭维,杨德莲,张婕,熊本海,蒋林树..采用Illumina MiSeq测序技术分析葡萄籽原花青素对奶牛体外瘤胃发酵产甲烷菌区系的影响[J].动物营养学报,2019,31(1):314-323,10.基金项目
国家自然科学基金项目 (31772629, 31702302) （31772629, 31702302）
2015年大北农青年教师科研基金 （）
2016年北京农学院青年教师科研基金 （）
2017年北京市教委科技计划项目 (SQKM201710020011) （SQKM201710020011）
"十三五"国家重大科技专项 (2016YFDO700201) （2016YFDO700201）
北京市农业局北京市现代农业产业技术体系奶牛创新团队 （）
