动物营养学报2019，Vol.31Issue(1)：477-484,8.DOI:10.3969/j.issn.1006-267x.2019.01.056
不同长度的饲用苎麻青贮对黑山羊瘤胃发酵参数及纤维素酶活性的影响
Effects of Different Lengths of Feeding-Ramie Silage on Rumen Fermentation Parameters and Cellulase Activities of Black Goats
摘要
Abstract
This experiment was conducted to investigate the effects of different lengths of feeding-ramie silage on rumen fermentation parameters and cellulase activities of black goats.Twelve black goats with similar body weight [ (20.35±2.16) kg]were randomly divided into 4 groups with 4 goats per group.Goats in the 4 groups were fed total mixed rations (TMRs) which used the feeding-ramie silage as a roughage, and the lengths of feeding-ramie silage were 1, 2 and 3 cm, respectively.On the 11 th day of feeding, the rumen contents were collected by the oral cavity at 0.5, 2.0 and 6.0 h after morning feeding, and the rumen fermentation parameters and cellulase activities were measured.The results showed as follows:1) the length of feeding-ramie silage had no significant effect on average daily intake of goats (P>0.05) .The pH in rumen fluid of 1 cm group was significantly lower than that of 2 and 3 cm groups at 0.5 and 2.0 h after morning feeding (P<0.05) .The rumen fluid ammonia nitrogen concentration of 1 cm group was the lowest, and it significantly lower than that of 2and 3 cm groups at 2.0 h after morning feeding (P<0.05) .2) The length of feeding-ramie silage had significant effects on the acetic acid to propionic acid ratio (P=0.026) , especially the acetic acid to propionic acid ratio of 3 cm group was lower than that of other two groups at all time points, but there were no significant effects on individual volatile fatty acids (VFAs) and total VFAs (P>0.05) .3) The length of feeding-ramie silage had no significant influences on cellulose activities in rumen fluid (P>0.05) , but the sampling time had a significant effect on β-glucuronidase, pectinase and xylanase activities in rumen fluid (P<0.05) , and the feedingramie silage length and sampling time had significant interaction effects on carboxymethyl cellulase and xylanase activities in rumen fluid (P<0.05) .In conclusion, the feeding-ramie silage with 3 cm chopped length is more conducive to rumen fermentation of black goats under this experiment conditions.关键词
饲用苎麻/青贮/瘤胃发酵/纤维素酶活性Key words
feeding-forage ramie/silage/rumen fermentation/cellulase activities分类
农业科技引用本文复制引用
孙盛楠,林谦,向海,侯振平,钟荣珍,戴求仲,吴端钦..不同长度的饲用苎麻青贮对黑山羊瘤胃发酵参数及纤维素酶活性的影响[J].动物营养学报,2019,31(1):477-484,8.基金项目
国家自然科学基金项目 (31501988) （31501988）
湖南省重点研发计划 (2016NK2170) （2016NK2170）
