南方医科大学学报2019，Vol.39Issue(2)：222-226,5.DOI:10.12122/j.issn.1673-4254.2019.09.15
产前应激子代大鼠海马Wnt/β-catenin信号通路的改变诱导抑郁和焦虑样行为
Changes of Wnt/β-catenin signaling pathway in the hippocampus caused by prenatal stress induce depression-and anxiety-like behaviors in rats
摘要
Abstract
Objective To explore the pathogenic role of changes of Wnt/β-catenin signaling pathway in the hippocampus in depression-and anxiety-like behaviors caused by prenatal stress (PS) in offspring rats.Methods Twelve female SpragueDawley rats weighing 240-260 g were randomly divided into control and restraint stress groups.The rats in the control group received no interventions, and those in restraint stress group were subjected to restraint stress (three times a day, 45 min each time) at the gestational age of 14-20 days.The 1-month-old offspring rats underwent open field test and forced swimming test to assess the anxiety-and depression-like behaviors, and the expressions of Wnt1, Gsk-3β and β-catenin in the hippocampus were detected using Western blotting.Results In open field test, the offspring rats with PS showed significantly decreased crossings of the center (P＜0.01) with reduced time spent in the center (P＜0.05) compared with control offspring rats.In forced swimming test, the offspring rats in PS group exhibited a significantly longer immobility time than in the control rats, and showed obvious depression-and anxiety-like behaviors.Compared with those in the control offspring rats, Gsk-3β expression increased significantly while the expressions of β-catenin and Wnt1 were significantly lowered in the hippocampus of the offspring rats in PS group (P＜0.01).Conclusion PS causes changes in Wnt/β-catenin signaling pathway in the hippocampus to contribute to the occurrence of depression-and anxiety-like behaviors in rats.关键词
产前应激/子代大鼠/海马/糖原合成酶激酶-3 (Gsk-3β)/β-cateninKey words
prenatal stress/offspring rats/hippocampus/glycogen synthase kinase 3/β-catenin引用本文复制引用
张玉荣,王瑞忠,陈蕊,王莉..产前应激子代大鼠海马Wnt/β-catenin信号通路的改变诱导抑郁和焦虑样行为[J].南方医科大学学报,2019,39(2):222-226,5.基金项目
陕西省教育厅科研计划 (16JK1643) （16JK1643）
