中国耳鼻咽喉头颈外科2019，Vol.26Issue(1)：5-8,4.DOI:10.16066/j.1672-7002.2019.01.002
Aurora-A促进鼻咽癌化疗抵抗的机制研究
Mechanism of Aurora-A to promote chemoresistance in nasopharyngeal carcinoma
摘要
Abstract
OBJECTIVE To explore the mechanism of Aurora kinase A (Aurora-A) promoting cancer cell chemotherapy resistance in nasopharyngeal carcinoma. METHODS The expression of Aurora-A in nasopharyngeal carcinoma tissues and adjacent tissues were detected by Western bolt and Q-PCR. The highexpressing Aurora A cell line CNE2 was used to detected the cell apoptosis and the expression of key pathway marker protein after Aurora-A inhibitor VX680 and cisplatin treatment by using Flow cytometry and WB. RESULTS The expression of Aurora-A in nasopharyngeal carcinoma tissues was significantly higher than that in adjacent tissues. Comparing to normal nasopharyngeal cells NP69, Aurora-A was significantly highly expressed in all of nasopharyngeal carcinoma cells and was highest in CNE2. Inhibiton of Aurora-A increased the cell apoptosis and the expression of p-AKT, p21 and Cleaved-Caspase-3 after using cisplatin or the Aurora-A inhibitor VX680 treatment. CONCLUSION The results shown that Aurora-A confer chemoresistance to cisplatin treatment through p-AKT/p21/Cleaved-Caspase-3 pathway.关键词
鼻咽肿瘤/细胞凋亡/顺铂/化疗抵抗/丝/苏氨酸蛋白激酶Key words
Nasopharyngeal Neoplasms/Apoptosis/Cisplatin/chemotherapy resistance/Aurora kinase A引用本文复制引用
范小琴,宋健,王雨洁,吴汉伟,陆璐,聂国辉..Aurora-A促进鼻咽癌化疗抵抗的机制研究[J].中国耳鼻咽喉头颈外科,2019,26(1):5-8,4.基金项目
中国博士后基金(2018M622876、2017M622877)和深圳市科技创新委员会项目(JCYJ20170306091657539、JCYJ20170306091452714、JCYJ20170302165727389)联合资助 （2018M622876、2017M622877）
