法医学杂志2018，Vol.34Issue(6)：606-610,5.DOI:10.12116/j.issn.1004-5619.2018.06.006
人体尿液中卡西酮类毒品的SPE-GC-MS定性定量分析
Qualitative and Quantitative Analysis of Cathinones in Human Urine by SPE-GC-MS
王平 1刘晓云 2刘遥 1罗叶锋 2王震3
作者信息
- 1. 广州正孚检测技术有限公司, 广东 广州 510663
- 2. 广东正孚法医毒物司法鉴定所, 广东 广州 510663
- 3. 广州万孚生物技术股份有限公司, 广东 广州 510663
- 折叠
摘要
Abstract
Objective To propose an alternative solid phase extraction-gas chromatography-mass spectrometry (SPE-GC-MS) method for the sensitive determination of cathinones in human urine samples, plus methodological verification.Methods Human urine samples were concentrated by solid phase extraction (SPE) sorbent and converted into the corresponding heptafluorobutyric acid anhydride derivatives.Methcathinone, 4-methyl methcathinone and 3, 4-methylenedioxy-N-methylcathinone were detected by gas chromatography-mass spectrometry (GC-MS).With quantitative analysis by internal standard method, methodological verification was carried out from the aspects of specificity, precision and recovery rate.Results Methcathinone, 4-methyl methcathinone and 3, 4-methylenedioxy-N-methylcathinone showed good linearly in the range of 25-200 ng/mL of urine (r＞0.99), with the limits of detection2.0 ng/mL, limit of quantitation 25.0 ng/mL, both intra-and inter-day coefficients of variation lower than 6.0％, and recovery rates of 98.4％-105.7％.Conclusion The proposed SPE-GC-MS procedure has good accuracy and specificity, can meet the need of qualitative and quantitative analysis of cathinones in the urine of drug abusers, and therefore provide technical support for the detection of cathinone abuse.关键词
法医毒理学/固相萃取/气相色谱-质谱法/甲卡西酮/4-甲基甲卡西酮/3,4-亚甲二氧基甲卡西酮/尿液Key words
forensic toxicology/solid phase extraction/gas chromatography-mass spectrometry/methcathinone/4-methyl methcathinone/3, 4-methylenedioxy-N-methylcathinone/urine分类
社会科学引用本文复制引用
王平,刘晓云,刘遥,罗叶锋,王震..人体尿液中卡西酮类毒品的SPE-GC-MS定性定量分析[J].法医学杂志,2018,34(6):606-610,5.
