广东医学2019，Vol.40Issue(1)：80-86,7.DOI:10.13820/j.cnki.gdyx.20182609
miR-23b通过Smad3信号通路抑制TGF-β1诱导的气道平滑肌细胞增殖的研究
miR-23b inhibits the proliferation of airway smooth muscle cells induced by TGF-β1 via Smad3 signal pathway
摘要
Abstract
Objective To investigated the effects and mechanism of miR-23b on the proliferation of airway smooth muscle cells(ASMCs)proliferation. Methods Primary ASMCs from BALB/c mice were isolated and cultured by tissue-piece inoculation, and then passaged. After ASMCs were transfected with miR-23b mimics or miR-23b inhibitor, TGF-β1(10 μg/L)were added to the transfected ASMCs, and the effects of miR-23b and TGF-β1 on ASMCs proliferation and apoptosis were evaluated through CCK8 and flow Cytometry, respectively. The target genes of miR-23b were predicted via bioinformatics software. Meanwhile, the expression of Smad3, which is the target gene of miR-23b, was determined using RT-PCR and Western blot. Results The overexpression of miR-23b significantly inhibited proliferation of ASMCs which induced by TGF-β1, and obviously promote the apoptosis. In addition, we also found that miR-23b negatively regulated the expression of Smad 3. Conclusion miR-23b could suppress the proliferation of ASMCs induced by TGF-β1 via Smad3 signaling pathway.关键词
miR-23b/TGF-β1/Smad3/气道平滑肌细胞Key words
miR-23b/Smad3/airway smooth muscle cells分类
医药卫生引用本文复制引用
张炜,黄林洁,陈茗..miR-23b通过Smad3信号通路抑制TGF-β1诱导的气道平滑肌细胞增殖的研究[J].广东医学,2019,40(1):80-86,7.基金项目
国家自然科学基金资助项目(编号:81500018) （编号:81500018）
广东省自然科学基金资助项目(编号:2016A030313332) （编号:2016A030313332）
