广东医学2019，Vol.40Issue(1)：95-100,6.DOI:10.13820/j.cnki.gdyx.20184336
结直肠癌SOS1、K-ras共表达对预后的影响
Co-expression of SOS1 and k-ras in prediction of prognosis of colorectal cancer
摘要
Abstract
Objective To investigate the expression and clinical significance of K-ras protein and SOS1 protein in human colorectal cancer tissues. Methods Specimens of 180 colorectal cancer tissues and 45 para-carcinoma tissues were collected from Henan Cancer Hospital. The expression of the K-ras and SOS1 proteins in both colorectal cancer and para-carcinoma tissues were assessed. Based on the results of immunohistochemistry, the correlation between the expression of K-ras and SOS and clinicopathological variables as well as prognosis were analyzed. Results K-ras protein was observed in the cell membrane and cytoplasm. There was a significant difference between the expression of K-ras protein in colorectal cancer tissues and para-carcinoma tissues(P = 0.001). The positive rate of K-ras in colorectal cancer tissues was 78.8％, and 53.3％ in para-carcinoma tissues. SOS1 expression was only observed in the cytoplasm. The positive rate of SOS in colorectal cancer tissues was 50.6％, but 28.9％ in para-carcinoma tissues. There was also a significant difference between the expression of SOS1 protein in colorectal cancer tissues and para-carcinoma tissues(P =0.007). Significant correlation of K-ras protein overexpression was observed with lymph node metastasis(P = 0.002)and TNM stage(P = 0.013). Whereas, no significant correlation was observed with age, differentiation, tumor site or invasion. There was no significant correlation between the expression of SOS1 protein and clinicopathological variables. The4 years overall survival(OS)of patients with positive K-ras and positive SOS1 was significantly shorter than that of positive K-ras and negative SOS1(HR = 2.381, 95％ CI: 1.103-5.137, P = 0.034). COX multivariate analysis showed that tumor T stage(HR = 2.118, 95％ CI: 1.109-4.046, P = 0.023), tumor N stage(HR = 2.487, 95％ CI: 1.103-5.610, P = 0.028)and immunohistochemical grouping(HR = 2.795, 95％ CI: 1.272-6.139, P = 0.010)were indepent risk factors of poor prognosis of patients with colorectal cancer. Conclusion K-ras protein may be involved in the progression of colorectal cancer and patients, and both positive K-ras protein and SOS1 suggested a worse prognosis.关键词
结直肠癌/K-ras蛋白/SOS1蛋白/预后Key words
colorectal cancer/K-ras protein/SOS1 protein/prognosis分类
医药卫生引用本文复制引用
曹建,万相斌,杜记涛,赵卫杰,陈广龙,赵智力,董志闯,孙淼淼,李智..结直肠癌SOS1、K-ras共表达对预后的影响[J].广东医学,2019,40(1):95-100,6.基金项目
河南省科技攻关项目(编号:182102310127) （编号:182102310127）
