广东药科大学学报2019，Vol.35Issue(1)：1-4,4.DOI:10.16809/j.cnki.2096-3653.2018111601
1株红树林可口革囊星虫共附生放线菌抑制COX-2活性成分的研究
Study on the chemical constituents with COX-2 inhibitory activity from a mangrove Phascolosoma esculenta symbiotic actinomycete
摘要
Abstract
Objective To isolate and screen Phascolosoma esculenta symbiotic actinomycetes from mangrove for COX-2 inhibitory activity, and study secondary metabolites from the active strain PE05. Methods The symbiotic actinomycetes were isolated using dilution-spreading-plate method. The COX-2 inhibitory activities of fermentation products from all of strains were evaluated by COX inhibitor screening assay kit. PE05 was confirmed as the target strain and isolated by various column chromatography and recrystallization. Moreover, the structures of pure compounds were identified by spectroscopic methods. Results Eleven symbiotic actinomycetes were isolated from Phascolosoma esculenta, and six of them showed COX-2 inhibitory activities. Five compounds were obtained from PE05, which were identified as vanillic acid (1), 4-hydroxybenzoie acid (2), phenylethyl acid (3), nicotinic acid (4), cyclo- (Pro-Leu) (5). Conclusion PE05 of Phascolosoma esculenta could be a suitable candidate as a potential COX-2 inhibitor producer, which will provide a new strategy for COX-2 inhibitor discovery.关键词
红树林/共附生放线菌/次级代谢产物/可口革囊星虫/COX-2抑制剂Key words
mangrove/symbiotic actinomycetes/secondary metabolites/Phascolosoma esculenta/COX-2 inhibitors分类
医药卫生引用本文复制引用
叶子聪,覃翠颖,陈钢,温露..1株红树林可口革囊星虫共附生放线菌抑制COX-2活性成分的研究[J].广东药科大学学报,2019,35(1):1-4,4.基金项目
国家自然科学基金项目(81502944) （81502944）
