广东药科大学学报2019，Vol.35Issue(1)：33-36,4.DOI:10.16809/j.cnki.2096-3653.2018121001
正交试验法优化铁皮石斛多糖的高效高压差低温连续式提取工艺
Study on high-efficiency, high-pressure differential and low temperature continuous extraction (hhlse) technology of polysaccharides from Dendrobium candidum by orthogonal test
摘要
Abstract
Objective To optimize the high-efficiency, high-pressure differential and low temperature continuous extraction, isolation and concentration technology and complete equipment to extract polysaccharides from Dendrobium candidum in pilot test and the medium test, in order to provide a reference for large-scale production. Methods With the extraction rate of polysaccharides from Dendrobium candidum as the evaluation index, the orthogonal test L9 (34) was used to investigate the three factors which influenced the extraction process of polysaccharides from Dendrobium candidum, such as grinding degree, ratio of dried powder of Dendrobium candidum to water and extraction pressure. Results The optimal parameters of extraction process were as follows: grinding degree of 1.2 mm, 1:25 (g:mL) ratio of dried powder of Dendrobium candidum to water, extraction pressure of 40 MPa. Under the conditions, the extraction rate of polysaccharides was 48.5％, and the extraction rate of medium test was 45.0％. Conclusion The extraction technology optimized by orthogonal test is stable, simple and high extraction rate, which has a certain guiding significance for the large-scale production of polysaccharides from Dendrobium candidum.关键词
铁皮石斛/多糖/高压差/中试试验/低温水提Key words
Dendrobium candidum/polysaccharide/high-pressure differential/medium test/low temperature water extraction分类
医药卫生引用本文复制引用
宋力飞,刘常青,马志鹏,王旭龙,罗俊,容永赐,李伟杰..正交试验法优化铁皮石斛多糖的高效高压差低温连续式提取工艺[J].广东药科大学学报,2019,35(1):33-36,4.基金项目
国家重点研发计划项目(2017YFC1703100) （2017YFC1703100）
