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HPLC法测定连翘不同部位中罗汉松树脂酚苷和牛蒡子苷及其苷元的含量

高纳影 贾秀娟 孙华庚 赵艳敏 刘岱琳 高晓霞

广东药科大学学报2019,Vol.35Issue(1):37-42,6.
广东药科大学学报2019,Vol.35Issue(1):37-42,6.DOI:10.16809/j.cnki.2096-3653.2018121404

HPLC法测定连翘不同部位中罗汉松树脂酚苷和牛蒡子苷及其苷元的含量

Determination of matairesinoside, matairesinol, arctiin and arctigenin in Forsythiae by HPLC

高纳影 1贾秀娟 2孙华庚 3赵艳敏 4刘岱琳 4高晓霞1

作者信息

  • 1. 广东药科大学药学院,广东 广州 510006
  • 2. 山东东阿阿胶股份有限公司,山东 聊城 252299
  • 3. 天津市尖峰天然产物研究开发有限公司,天津 300309
  • 4. 中国人民武装警察部队后勤学院生药学与药剂学教研室,天津 300162
  • 折叠

摘要

Abstract

Objective To determine the contents of matairesinoside, matairesinol, arctiin, and arctigenin in different parts of Forsythia suspensa. Methods High performance liquid chromatography was used to determine the contents of matairesinoside, matairesinol, arctiin, and arctigenin. The mobile phase was acetonitrile-water with gradient elution, the detection wavelength was 220 nm, the flow rate was 1 mL/min, and the column temperature was 30 ℃. Results The linear relationships of matairesinoside, matairesinol, arctiin, and arctigenin were respectively good with R2=0.999 9. The contents of matairesinoside, matairesinol, arctiin and arctigenin in the fruit of Forsythia suspensa were 0.044%, 0.078%, 0.35% and 0.018%, respectively. The contents of matairesinoside, matairesinol, arctiin and arctigenin were 0.98%, 0.44%, 0.67%, and 0.022%, respectively in the stem of Forsythia suspensa, 1.85%, 0.63%, 0.35%, and 0.088%, respectively in the branch of Forsythia suspensa, and 0.31%, trace, 3.93% and 0.22% respectively in the leaf of Forsythia suspensa. Conclusion The contents of matairesinoside, matairesinol in the branches of Forsythia suspensa were relatively higher than others, while the contents of arctiin and arctigenin in leaves of Forsythia suspensa were relatively higher. The distribution of four components in different parts of Forsythia suspensa has been studied, which provides experimental basis for the follow-up study of Forsythia suspensa and the development of health products.

关键词

连翘/罗汉松树脂酚苷/罗汉松树脂酚/牛蒡子苷/牛蒡子苷元

Key words

Forsythia suspensa/matairesinoside/matairesinol/arctiin/arctigenin

分类

医药卫生

引用本文复制引用

高纳影,贾秀娟,孙华庚,赵艳敏,刘岱琳,高晓霞..HPLC法测定连翘不同部位中罗汉松树脂酚苷和牛蒡子苷及其苷元的含量[J].广东药科大学学报,2019,35(1):37-42,6.

广东药科大学学报

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1006-8783

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