广东药科大学学报2019，Vol.35Issue(1)：53-58,6.DOI:10.16809/j.cnki.2096-3653.2018091401
芦根提取物与苇根提取物HPLC特征图谱的比较
Study on HPLC characteristic spectra of Phragmitis Rhizoma extract and Zizaniae caduciflorae Rhizoma extract
谭丽容 1周楚娟 2程敏 1黄月纯 3张龙开 1魏刚 4王雅文2
作者信息
- 1. 无限极(中国)有限公司,广东 江门 529156
- 2. 广州中医药大学第一临床医学院,广东 广州 510405
- 3. 广州中医药大学第一附属医院,广东 广州 510405
- 4. 广州中医药大学中药学院,广东 广州 510006
- 折叠
摘要
Abstract
Objective To compare the difference of HPLC characteristic spectra of Phragmitis Rhizoma extract and Zizaniae caduciflorae Rhizoma extract, so as to identify the Phragmitis Rhizoma extract and its adulterants. Methods HPLC was performed on ZORBAX SB C18 column (250 mm×4.6 mm, 5 μm), the acetonitrile-0.5％ potassium dihydrogen phosphate aqueous solution (pH2.15) was employed as a mobile phase at gradient elution, detection wavelength was 275 nm;column temperature was 30℃, and flow rate was 1.0 mL/min. The contrast spectrum was generated with Traditional Chinese medicine (TCM) chromatographic fingerprint similarity evaluation software system (2004 A version) by State Pharmacopoeia Commission. Results Seven common peaks were separated from 10 batches of Phragmitis Rhizoma extract, including p-coumaric acid and ferulic acid. Thirteen common peaks were separated from 10 batches of Zizaniae caduciflorae Rhizoma extract, which included 7 same common peaks of Phragmitis Rhizoma extract and six common peaks (peak w1-peak w6) of flavonoids easily recognized. The similarities of 10 batches of Phragmitis Rhizoma extract and Zizaniae caduciflorae Rhizoma extract were 0.935-0.999, 0.807-0.973 respectively, and the similarity of the mutual modes between Phragmitis Rhizoma extract and Zizaniae caduciflorae Rhizoma extract was 0.875. Conclusion The method is accurate, reliable and reproducible, which can be used for the identification and quality evaluation of Phragmitis Rhizoma extract.关键词
芦根提取物/苇根提取物/特征图谱/高效液相色谱法Key words
Phragmitis Rhizoma extract/Zizaniae caduciflorae Rhizoma extract/characteristic spectrum/HPLC分类
医药卫生引用本文复制引用
谭丽容,周楚娟,程敏,黄月纯,张龙开,魏刚,王雅文..芦根提取物与苇根提取物HPLC特征图谱的比较[J].广东药科大学学报,2019,35(1):53-58,6.
