广东药科大学学报2019，Vol.35Issue(1)：76-81,6.DOI:10.16809/j.cnki.2096-3653.2018120403
丹参提取物及丹酚酸B对庆大霉素耳毒性保护作用的体内外研究
Protective effect of Salvia miltiorrhiza extract and salvianolic acid B against gentamicin-induced ototoxicity in vitro and in vivo
摘要
Abstract
Objective To investigate the protective effects of Salvia miltiorrhiza extract and salvianolic acid B (Sal B) against drug-induced hearing loss (DIHL). Methods HEI-OC1 cells and zebrafish were exposed to gentamicin after pretreated by Salvia miltiorrhiza extract or Sal B. The viability of HEI-OC1 cells was analyzed by MTT assay and the damage of the zebrafish hair cells was detected using DASPEI staining. Results In HEI-OC1 cell experiment, the cell viability of Salvia miltiorrhiza extract or Sal B group was higher than that of gentamicin group (P<0.01). In the zebrafish hair cell experiment, the hair cell fluorescence intensity in Salvia miltiorrhiza extract group was more than that of gentamicin group (P<0.01), but Sal B group had no significant difference. Conclusion Salvia miltiorrhiza extract has protective effects against DIHL, and Sal B will be a potential ototoxic therapeutic agent after improving its stability.关键词
丹参提取物/丹酚酸B/HEI-OC1细胞/斑马鱼/耳毒性Key words
Salvia miltiorrhiza extract/salvianolic acid B/HEI-OC1 cells/zebrafish/ototoxicity分类
医药卫生引用本文复制引用
吴艾欣,李娟,陈钢,温露..丹参提取物及丹酚酸B对庆大霉素耳毒性保护作用的体内外研究[J].广东药科大学学报,2019,35(1):76-81,6.基金项目
国家自然科学基金项目(81502944 （）
81773917) （）
