广东药科大学学报2019，Vol.35Issue(1)：88-91,4.DOI:10.16809/j.cnki.2096-3653.2018112108
萝卜硫素对人胃癌细胞HGC27的抑制作用及其机制研究
Study on the inhibitory effects of sulforaphane on human gastric cancer cells and its mechanism
冯莉芳 1涂毅 2张玲莉2
作者信息
- 1. 武汉大学人民医院感染管理办公室,湖北 武汉 430060
- 2. 武汉大学人民医院药学部,湖北 武汉 430060
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effects of sulforaphane on human gastric cancer cells (HGC27) and its possible mechanism. Methods HGC27 cells were divided into four groups:sulforaphane low dose, middle dose and high dose groups (20, 40 and 80 μg/mL) and the control group. CCK8 assay was used to detect the survival rate of HGC27 cells. The levels of reactive oxygen species (ROS) and adenosine triphosphate (ATP) were measured using DCFH-DA and HPLC, respectively. Western blot was performed to analyze the expression of Bax, Bcl-2 and p53. Results With different concentrations of sulforaphane treatment, the relative survival rates of HGC27 cells decreased in a dose-and time-dependent manner (P<0.05). ROS and ATP content decreased significantly (P<0.05). The expression levels of Bax and p53 were increased, but the expression of Bcl-2 was decreased significantly (P<0.05). Conclusion Sulforaphane can inhibit the growth of HGC27 cells, and the possible mechanism may be related with inhibition of ROS and ATP generation, upregulation of Bax and p53 expression and downregulation of Bcl-2 expression.关键词
萝卜硫素/人胃癌细胞/存活率/机制Key words
sulforaphane/HGC27/survival rate/mechanism分类
医药卫生引用本文复制引用
冯莉芳,涂毅,张玲莉..萝卜硫素对人胃癌细胞HGC27的抑制作用及其机制研究[J].广东药科大学学报,2019,35(1):88-91,4.
