广东药科大学学报2019，Vol.35Issue(1)：120-125,6.DOI:10.16809/j.cnki.2096-3653.2018110503
产纤维素酶细菌的分离、鉴定与酶学性质研究
Isolation and identification of cellulase-producing strains and characterization of their cellulases
摘要
Abstract
Objective To provide strain resources for the comprehensive utilization of cellulose resources by screening of cellulase-producing strains and studying of the enzymatic properties of cellulase. Methods CMC-Congo red staining and enzyme activity assay were used to screen the cellulase-producing strains from compost of traditional Chinese herbal tea residue in Guangzhou and soil of cornfields in Heilongjiang province. 16 S rDNA sequence analysis was used for gene identification of the strains. Single factor tests were used to determine influence of temperature, pH, metal ions, organic solvents and salt concentration on the cellulase activity and stability. Results 3 strains of Bacillus Z-1, Z-2 and H-3 were screened out, and their cellulase activities were 977.92 U/mL, 1 089.79 U/mL, and 1 538.44 U/mL, respectively. The optimal reaction temperature was 50 ℃, and the optimal pH was pH 4.0～5.0. The cellulases were activated by Ag+, Co2+, Mg2+, Tris-100, and methanol in a certain concentration range. The relative cellulase activity still reached to 56.34％ under 1.5 mol/L sodium chloride solution treatment for 4 h. Conclusion The cellulases produced by B. methylotrophicus Z-1, B. subtilis Z-2, and B. subtilis H-3 are resistant to heat, acid and salt, and can be activated by some metal ions and organic substances, which exhibit the potential development value.关键词
纤维素酶产生菌/菌种筛选/16S rDNA/酶学性质Key words
cellulase-producing strain/strain screening/16S rDNA/enzymology properties分类
医药卫生引用本文复制引用
梁倩,李荷,王卓娅..产纤维素酶细菌的分离、鉴定与酶学性质研究[J].广东药科大学学报,2019,35(1):120-125,6.基金项目
广东省大学生创新创业训练计划项目(201610573030) （201610573030）
广东省科技计划项目(2017A010105011) （2017A010105011）
广州市科技计划项目(201802030009) （201802030009）
