广西医科大学学报2019，Vol.36Issue(4)：495-500,6.DOI:10.16190/j.cnki.45-1211/r.2019.04.001
高糖高脂环境下生长激素释放肽对胰腺β细胞自噬的影响
Effect of ghrelin on autophagy of pancreatic β cells under high glucose and high lipid environment
袁雪 1廖婷 1荣曦 1刘红1
作者信息
- 1. 广西医科大学第一附属医院老年内分泌代谢科,南宁 530021
- 折叠
摘要
Abstract
Objective:To investigate the effect of ghrelin on the autophagy of islet β cells under high glucose and high lipid (HGHL) environment.Methods:Islet NIT-1cells were cultured and divided into four groups:control group(glucose 5.6 mmol/L) ,ghrelin group(glucose 5.6mmol/L+100nmol/L ghrelin) ,HGHL group(glucose 33.3mmol/L+0.5mmol/L palmitic acid) ,HGHL+ghrelin group (glucose 33.3mmol/L+0.5mmol/L PA+100nmol/L ghrelin).The cell counting kit (CCK-8) was used to assess the NIT-1cells viability. Monodansylcadaverine(MDC) staining and microtubule-associated protein 1light chain 3 (LC3) B immunofluorescence staining were employed to detect the autophagy.Western blotting was performed to determine the protein expression of Beclin 1and the ratio of LC3 Ⅱ to LC3Ⅰ,and real-time fluorescence quantitative PCR(qPCR) was tomeasure the mRNA expression of Atg6 and LC3B.Results:The cell viability,the protein level of Beclin 1,the ratio of LC3 Ⅱ/LC3 Ⅰ,and the mRNA expression levels of Atg 6 and LC3B in HGHL+ghrelin groupweresignificantly increased compared with HGHL group (P<0.05).MDC staining and immunofluorescence staining also showed increased deposition of autophagic vesicles and autophagic protein LC3B in HGHL+ghrelin group. Conclusion:Ghrelin could protect islet β cells from HGHL injury, which might be related to the enhancement of autophagy.关键词
ghrelin/胰岛β细胞/自噬/高脂高糖Key words
ghrelin/islet β cell/autophagy/HGHL分类
医药卫生引用本文复制引用
袁雪,廖婷,荣曦,刘红..高糖高脂环境下生长激素释放肽对胰腺β细胞自噬的影响[J].广西医科大学学报,2019,36(4):495-500,6.
