广西医科大学学报2019，Vol.36Issue(4)：501-506,6.DOI:10.16190/j.cnki.45-1211/r.2019.04.002
长期静脉注射羧基化单壁碳纳米管对大鼠肾脏的毒性作用研究
Toxic effect of long-term intravenous injection of carboxylated single-walled carbon nanotubes on kidney in rats
摘要
Abstract
Objective:To evaluate the toxic effect of long-term intravenous injection of carboxylated singlewalled carbon nanotubes(c-SWNTs) on kidney in rats.Methods:Sixty-four rats were divided randomly into control group and c-SWNTs group,with 32cases in each group.Rats in the control group were injected with 5％ glucose solution via tail vein,and rats in c-SWNTs group were injected with c-SWNTS-glucose solution via tail vein.After 30d,60d,90d and 120d(90 dinjection+30 drecovery) of intervention,eight rats were selected from each group and killed after anesthesia.Ultrastructure of renal tissue was observed by transmission electron microscope(TEM).Hematoxylin-eosin(HE) staining and Masson staining were used to observe pathological changes of renal tissue.Western blotting was used to detect the expression of type Ⅲ collagen(Col Ⅲ) protein.Results:After 30 days of intervention,reduced glomerular volume,karyopyknosis and swollen mitochondria were observed in the c-SWNTs group.Locally thickened renal vessels walls,glomerular capillary loop adhered to Bowman's capsule, inflammatory cell infiltration in capillary loop and spread to periglomerular interstitium with the prolong of treatment time,obvious dilation of renal tubule lumen,and periglomerular and medulla interstitial fibrogenesis were found in the c-SWNTs group by HE staining and Masson staining.After 90 days,the rats in the c-SWNTs group had severest renal inflammation and fibrosis,and significantly higher protein level of ColⅢcompared with the control group(P<0.05).There was no significant difference in the protein expression of ColⅢafter 30days of recovery between control group and c-SWNTs group(P>0.05).Conclusion:Long-term intravenous injection of c-SWNTs might lead to renal cortex,renal medulla and renal vessels injuries,and reversible inflammatory response and renal fibrosis.关键词
单壁碳纳米管/静脉注射/肾脏/炎性反应/纤维化Key words
single-walled carbon nanotubes/intravenous injection/kidney/inflammatory response/fibrosis分类
医药卫生引用本文复制引用
谢雪平,成晓静,秦悦,李苏宁,刘华钢,赖泽锋..长期静脉注射羧基化单壁碳纳米管对大鼠肾脏的毒性作用研究[J].广西医科大学学报,2019,36(4):501-506,6.基金项目
国家自然科学基金资助项目(No.21367006) （No.21367006）
广西自然科学基金资助项目(No.2012GXNSFBA053112) （No.2012GXNSFBA053112）
广西教育厅科研课题资助项目(No.200103YB024) （No.200103YB024）
