广西医科大学学报2019，Vol.36Issue(4)：507-510,4.DOI:10.16190/j.cnki.45-1211/r.2019.04.003
龙蛭汤对大鼠脑缺血再灌注损伤后Bcl-2和Caspase-3蛋白表达的影响
Effects of Longzhi decoction on the expression of Bcl-2 and Caspase-3in cerebral ischemia/reperfusion injury rats
摘要
Abstract
Objective:To investigate the effects of Longzhi decoction on the expression of Bcl-2and Caspase-3 in cerebral ischemia/reperfusion injury rats.Methods:A total of 48SD rats were divided into four groups (n=12):sham-operated group,model group,nimodipine group(0.01g/kg·d-1) and Longzhi decoction group (13.60g/kg·d-1).After 6days of administration,the rat model of right cerebral ischemia/reperfusion injury was established by the modified Longa thread occlusion method.After 24hof operation,the neurologic deficit score was evaluated.Hematoxylin-eosin(HE) staining was used to observe the pathological changes of ischemic penumbra in brain tissue.2,3,5-triphenyltetrazolium chloride(TTC) staining was used to compare the infarct volume of each group.Immunohistochemistry was used to detect the expression of Bcl-2 and Caspase-3in neurons of ischemic brain tissue.Results:The neurologic deficit score of the model group was significantly higher than that of the sham-operated group(P<0.01).The volume of cerebral infarction was reduced in nimodipine group and Longzhi decoction group compared with the model group(P<0.05).The neuronal damage was severe in the model group,and the cell morphology of penumbra in Longzhi decoction group and nimodipine group was significantly improved.The positive rate of Bc1-2protein was remarkably higher in Longzhi decoction group and nimodipine group than that of the model group, while the Caspase-3positive rate was lower(P<0.05).Conclusion:Longzhi decoction could improve the symptoms of neurological deficits in rats with ischemic brain injury,reduce the volume of cerebral infarction and the damage of neurons.The mechanism might be related to the regulation of Bcl-2and Caspase-3 expressions.关键词
脑缺血再灌注/龙蛭汤/细胞凋亡/Bcl-2/Caspase-3Key words
cerebral ischemia reperfusion/Longzhi decoction/cell apoptosis/Bcl-2/Caspase-3分类
医药卫生引用本文复制引用
邹玲,陈永斌,刘启华,李南方,黄显雯..龙蛭汤对大鼠脑缺血再灌注损伤后Bcl-2和Caspase-3蛋白表达的影响[J].广西医科大学学报,2019,36(4):507-510,4.基金项目
国家自然科学基金资助项目(No.81560790) （No.81560790）
广西自然科学基金资助项目(No.2014GXNSFAA118277) （No.2014GXNSFAA118277）
