广西医科大学学报2019，Vol.36Issue(4)：520-523,4.DOI:10.16190/j.cnki.45-1211/r.2019.04.006
低剂量溴化乙锭建立低含量线粒体DNA大鼠骨髓来源间充质干细胞模型
Mitochondrial DNA-less bone mesenchymal stem cells produced by low-dose of ethidium bromide
摘要
Abstract
Objective:To investigate the effect of low-dose ethidium bromide on mitochondrial DNA (mtDNA) of bone mesenchymal stem cells(BMSC).Methods:BMSC was divided into control group,C-49d group(50ng/mL ethidium bromide+50μg/mL sodium pyruvate+50μg/mL uracil) and non-uracil C-49 d group(50ng/mL ethidium bromide).The morphology of BMSC was observed and the cell viability was measured.The expression of mitochondrial protein coding genes was detected by real-time fluorescence quantitative PCR(qPCR).Results:The C-49dgroup cells presented polyhedral shape,slower growth,shorter and flatter than the controls.The cell viability of the control group was higher than that of the C-49d group and non-uracil C-49dgroup(P<0.05).Non-uracil C-49dgroup had lower cell viability than the C-49 dgroup (P<0.05).The expression of mitochondrial protein coding genes(ND1,ND2,ND3,COX1, COX2,COX3and CYTB) in the C-49dgroup was significantly lower than that in the control group(P<0.05).Conclusion:Low-dose of ethidium bromide could inhibit the expression of mtDNA in BMSC,and successfully construct mtDNA-less BMSC model.关键词
间充质干细胞/线粒体DNA/溴化乙锭Key words
bone mesenchymal stem cell/mitochondrial DNA/ethidium bromide分类
医药卫生引用本文复制引用
何熠,衡淑君,郑立..低剂量溴化乙锭建立低含量线粒体DNA大鼠骨髓来源间充质干细胞模型[J].广西医科大学学报,2019,36(4):520-523,4.基金项目
国家自然科学基金资助项目(No.81760326) （No.81760326）
广西医疗卫生适宜技术开发与推广应用项目(No.S201670) （No.S201670）
