广西医科大学学报2019，Vol.36Issue(4)：529-533,5.DOI:10.16190/j.cnki.45-1211/r.2019.04.008
β-连环蛋白表达在慢性阻塞性肺疾病大鼠气道重建中的调控作用研究
Regulatory effect of β-catenin gene expression on airway remodeling in rats with chronic obstructive pulmonary disease
摘要
Abstract
Objective:To investigate the regulation of β-catenin gene expression in airway remodeling in rats with chronic obstructive pulmonary disease(COPD).Methods:40rats were randomly divided into COPD group and control group,with 20rats in each group.COPD rat model was established by intratracheal injection of lipopolysaccharide(LPS) and cigarette exposure.The levels of transfer growth factor-β1(TGF-β1) , platelet-derived growth factor(PDGF) and matrix metalloproteinase(MMP-9) in the small airway fibroblasts(FB) supernatant were detected by enzyme-linked immunosorbent assay.The expression of β-catenin protein in the small airway FB were determined by western blotting.Pearson correlation analysis was performed to analyze the association between the expression of β-catenin and the levels of TGF-β1,PDGF and MMP-9in the small airway BF.Results:The levels of TGF-β1,PDGF and MMP-9as well as the expression of β-catenin protein in the COPD group were significantly higher than those in the control group (P<0.05).The protein expression of β-catenin was positively correlated with TGF-β1,PDGF and MMP-9levels (r=0.243,0.249and 0.358,P<0.05).Conclusion:The levels of FB proliferation factors were high,and were positively correlated withβ-catenin protein expression.We speculated thatβ-catenin exerted a regulatory action in airway remodeling.关键词
β-连环蛋白/慢性阻塞性肺疾病/气道重建/调控作用Key words
β-catenin/chronic obstructive pulmonary disease/airway remodeling/regulation分类
医药卫生引用本文复制引用
袁霞,罗弦,杨义,葛正行..β-连环蛋白表达在慢性阻塞性肺疾病大鼠气道重建中的调控作用研究[J].广西医科大学学报,2019,36(4):529-533,5.基金项目
国家自然科学基金资助项目(No.81473533 （）
No.81260599) （）
