工业微生物2019，Vol.49Issue(1)：8-13,6.DOI:10.3969/j.issn.1001-6678.2019.01.002
Streptomyces sp. FIM-16-06产他克莫司摇瓶发酵条件的优化
Optimization of fermentation conditions of tacrolimus-producing strain Streptomyces sp. FIM-16-06
摘要
Abstract
In order to optimize the fermentation conditions for tacrolimus-producing strain Streptomyces sp. FIM-16-06, the single factor experiments and orthogonal experiments were adopted to investigate the liquid fermentation medium and culturing parameters of Streptomyces sp. FIM-16-06. The results revealed that the appropriate fermentation medium composition and optimal fermentation parameters were determined as follows: fermentation medium containing corn starch6.0％, glucose 2.0％, soybean flour 2.0％ and corn steep liquor 0.5％, while the initial pH 7.5, filling volume 50 m L/500 m L shake flask, cultivation time of seed 48 h, inoculum size 10％, shaker''s rotating speed 250 r/min, fermentation temperature 27 ℃ and fermentation time 7 d. The tacrolimus yield of Streptomyces sp. FIM-16-06 under the optimized fermentation conditions was 60％ higher than that of the original. The optimal fermentation conditions of Streptomyces sp.FIM-16-06 laid the foundation for further industrial fermentation.关键词
他克莫司/发酵/正交试验Key words
tacrolimus/fermentation/orthogonal experiment引用本文复制引用
邱观荣,张祝兰,严凌斌,张引,任林英,连云阳..Streptomyces sp. FIM-16-06产他克莫司摇瓶发酵条件的优化[J].工业微生物,2019,49(1):8-13,6.基金项目
福建省属公益类研究所基本科研专项(No.2017R1009-1) （No.2017R1009-1）
福州市科技计划项目(No.2017-G-91) （No.2017-G-91）
福州市科技计划项目(No.2018-G-48) （No.2018-G-48）
