工业微生物2019，Vol.49Issue(1)：14-20,7.DOI:10.3969/j.issn.1001-6678.2019.01.003
基于CRISPR/Cas9系统对干酪乳杆菌进行红色荧光蛋白标记
Red fluorescent protein labeling of Lactobacillus casei based on CRISPR/Cas9 system
摘要
Abstract
In this study, CRISPR/Cas9 system was used to label Lactobacillus casei LC2 W with red fluorescent protein (RFP) for investigating the distribution and colonization of Lactobacillus casei in intestinal tract and evaluating its function as probiotics. First, the plasmid pLCNICK-1628-RFP was constructed and was introduced into the receptor cells of LC2 W. Then, the LC2 W-1628 gene in genome of LC2 W was deleted and the RFP gene was inserted into the genome of LC2 W. Therefore, LC2 W could express RFP. The results showed that RFP could be expressed stably in Lactobacillus casei LC2 W by measuring the fluorescence intensity.关键词
干酪乳杆菌/LC2W/CRISPR/Cas9系统/红色荧光蛋白Key words
Lactobacillus casei/LC2W/CRISPR/Cas9 system/red fluorescent protein引用本文复制引用
张莹,宋馨,夏永军,艾连中,王光强..基于CRISPR/Cas9系统对干酪乳杆菌进行红色荧光蛋白标记[J].工业微生物,2019,49(1):14-20,7.基金项目
国家重点研发计划项目(2018YFD0501600) （2018YFD0501600）
