工业微生物2019，Vol.49Issue(1)：21-25,5.DOI:10.3969/j.issn.1001-6678.2019.01.004
三株散囊菌发酵茶叶的初步研究
Preliminary study on fermentation of tea by three Eurotium spp. fungi
摘要
Abstract
The Eurotium spp. can give special flavor and aroma to the fermented tea. In this research three Eurotium spp. fungi Aspergillus chevalieri E2 (E2), Aspergillus chevalieri E3 (E3) and Aspergillus cristatus E6 (E6) were used, which were previously isolated and identified from the Liupao tea produced in Guangxi. The experiments were conducted including their growth conditions on optimized liquid Czapek medium at different temperature, the quality analysis of tea under different sterilization methods before being fermented by these fungi, the polyphenols content, the total antioxidant capacity and the DPPH·free radical scavenging ability of their tea soup. The results showed that the growth conditions for three fungi were good in optimized liquid Czapek medium at 31 ℃ ～ 34 ℃. When the temperature for tea fermentation was at 28 ℃, three fungi grew well with the initial fermentation water content more than or equal to 20％. Among them, E2 set and hybrid fermentation set had the fastest growth speed. On the surface of the tea leaves, E2 produced large amount of golden ascocarp and olivine conidiophores; E3 nearly just produced olivine conidiophores; and E6 almost only produced golden ascocarp. The content of polyphenols in fermented tea soup was lower than that in unfermented tea soup and their antioxidative indexe was also decreased, this explained the fermentation accelerated the oxidation of antioxidants in tea.This research had an important reference value to the fermentation of tea by different Eurotium fungi from Liupao tea.关键词
谢瓦氏曲霉/冠突曲霉/六堡茶/茶叶发酵/茶多酚/抗氧化活性Key words
Aspergillus chevalieri/Aspergillus cristatus/Liupao tea/tea fermentation/tea polyphenol/antioxidative activity引用本文复制引用
陈皓睿,孙强,闻东明,刘鸿,邹克兴,申佩弘,龙章德..三株散囊菌发酵茶叶的初步研究[J].工业微生物,2019,49(1):21-25,5.基金项目
广西中烟有限责任公司科技项目:"金花菌在烟叶发酵醇化中的应用研究"(No.GXZYCX2017B003) （No.GXZYCX2017B003）
广西科技厅科技攻关项目(桂科攻15118006) （桂科攻15118006）
