工业微生物2019，Vol.49Issue(1)：26-32,7.DOI:10.3969/j.issn.1001-6678.2019.01.005
磁性纳米颗粒固定化乙醇脱氢酶的研究
Immobilization of alcohol dehydrogenase by magnetic nanoparticles
余带兵 1李红梅 1高露姣 2孙十凡 1周福欢1
作者信息
- 1. 上海理工大学 医疗器械与食品学院,上海 200093
- 2. 东海水产研究所,上海 200093
- 折叠
摘要
Abstract
The immobilization of alcohol dehydrogenase by magnetic Fe3 O4 nanoparticles surface modified with (3-aminopropyl) triethoxysilane (APTES) and glutaraldehyde was studied, and the effects of the immobilization conditions on the immobilization efficiency were investigated. The results showed that Fe3 O4@ SiO2 nanoparticles modified with amino and aldehyde groups still kept great water dispersibility and colloidal stability, which could be used as the immobilization carrier. The optimum immobilized conditions were screed by single factor optimization, including the optimum amount of enzyme of 11.3 U/100 mg, shaked speed of 150 r/min, the immobilized pH of 6.5, immobilized temperature of 5 ℃ ～15 ℃ and immobilization time of 45 min. Under the optimum conditions, immobilization rate could reach 60.2％.Compared with free enzyme, the immobilized enzyme had better temperature resistance and alkali resistance. The immobilization rate of the immobilized alcohol dehydrogenase still remained at about 57％ after used for 8 times, which showed a great operating stability. This study could provide technical basis for continuous production of NADH.关键词
磁性纳米颗粒/表面修饰/乙醇脱氢酶/固定化Key words
magnetic nanoparticles/surface modified/alcohol dehydrogenase/immobilization引用本文复制引用
余带兵,李红梅,高露姣,孙十凡,周福欢..磁性纳米颗粒固定化乙醇脱氢酶的研究[J].工业微生物,2019,49(1):26-32,7.
