工业微生物2019，Vol.49Issue(1)：44-49,6.DOI:10.3969/j.issn.1001-6678.2019.01.008
基于全细胞催化合成NADP重组细胞复合通透剂的筛选
Screening for composite penetrators of recombinant cells based on NADP synthesis of whole cell catalysis
孙十凡 1李红梅 1卢环环 1余带兵1
作者信息
- 1. 上海理工大学医疗器械与食品学院,上海 杨浦 200093
- 折叠
摘要
Abstract
The effects of chemical penetrators on NADP biosynthesis by recombinant E. coli BL21 (DE3) pET30α (+) -Nmnat were investigated. The single factor screening showed that four kinds of permeable agents such as Triton X-100, Tween 80, dimethyl sulfoxide and cetyltrimethyl ammonium bromide (CTAB) all could increase the catalytic yield of the whole cell, however, the difference was not significant. Then, the factorial design was used to investigate the composite effects of the four penetrators on the whole cell catalysis. Except that the interactions of Triton X-100 with Tween 80 and Triton X-100 with CTAB (P> 0.05), were not significant. All the other interaction items (P < 0.05) showed significant difference, and the amount of CTAB was only increased in the composite penetrator. The synergy of the composite system would be reduced, and the restriction would be increased, so it was not suitable for compound formulation. Finally, the central composite design was applied to obtain the best ratio of the penetrators. The results showed that when 1.64％Triton X-100, 2.53％ Tween 80 and 1.06％ dimethyl sulfoxide were combined, the predicted NADP yield could reach the maximum 77.46％ in accordance with the verified yield, which revealed that the experimental optimization model had good predictability. The result of optimization was 47％ higher than that of no penetrator. It indicated that optimized formula for composite penetrators was beneficial to improving the NADP biosynthesis by recombinant bacteria.关键词
析因设计/中心组合设计/复合通透剂/全细胞催化/NADPKey words
factorial design/central composite design/complex penetrators/whole cell catalysis/NADP引用本文复制引用
孙十凡,李红梅,卢环环,余带兵..基于全细胞催化合成NADP重组细胞复合通透剂的筛选[J].工业微生物,2019,49(1):44-49,6.
