河南农业大学学报2019，Vol.53Issue(2)：200-206,7.
蝴蝶兰花器官冰冻切片技术探讨
A study on Phalaenopsis floral organ cryo-sectioning technique
摘要
Abstract
A sucrose protection-liquid nitrogen cryo-sectioning technology system was established by slicing the ovary,petal,lip and gynostemium of Phalaenopsis. That is designed to make the relatively complete microstructure sections of Phalaenopsis organ section through vacuum the phalaenopsis organs after being treated with sucrose phosphate buffer protection solution,and then freezed by the liquid nitrogen,fixing, embedding,film slicing,observation,staining and photographing. The results showed that the optimal conditions for cryo-sectioning of Phalaenopsis were as follows: (1) Optimum treatment of quality scoresucrose phosphate buffer was 4％ sucrose in PBS for ovary,8％ sucrose in PBS for petal,4％ sucrose in PBS for lip,and 16％ sucrose in PBS for gynostemium; (2) The optimum slice thickness is ovary 15 μm,petal 10 μm,lip flap 20 μm and gynostemium 10 μm; (3) The best staining solution was red stain for the three organs,ovary,petal and lip,whereas for the gynostemium was a red-solid green stain solution. These four floral organs were not suitable for solid green dyeing alone used.关键词
蝴蝶兰/花器官/液氮冷冻/冰冻切片技术Key words
Phalaenopsis/floral organ/liquid nitrogen freezing/cryo-sectioning technique分类
农业科技引用本文复制引用
陆振芳,蒋素华,江敏,韩世明..蝴蝶兰花器官冰冻切片技术探讨[J].河南农业大学学报,2019,53(2):200-206,7.基金项目
郑州师范学院科技创新团队支持计划资助(兰花种质资源开发与产业化应用科研创新团队) （兰花种质资源开发与产业化应用科研创新团队）
贵州省教育厅项目(黔教合KY字[2017]012,黔教合KY字[2018]371) （黔教合KY字[2017]012,黔教合KY字[2018]371）
六盘水市科技局项目(52020 - 2017 - 02 - 03,52020 - 2018 - 02 - 14) （52020 - 2017 - 02 - 03,52020 - 2018 - 02 - 14）
