化工进展2019，Vol.38Issue(3)：1567-1572,6.DOI:10.16085/j.issn.1000-6613.2018-0952
低C/N比异养硝化-好氧反硝化菌筛选及硝化特性
Screening and nitrification characteristics of heterotrophic nitrificationaerobic denitrification bacteria with low C/N ratio
摘要
Abstract
In view of the problems of insufficient carbon source and low nitrogen removal efficiency for biological treatment of low C/N ratio wastewater, a heterotrophic nitrification-aerobic denitrification strain WUST-7 with low C/N ratio was isolated from the activated sludge of petrochemical wastewater treatment plant. Pseudomonas sp. was identified by morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis. The effects of carbon source type, culture temperature, initial pH and rotation speed on the nitrification characteristics were investigated by single factor experiments. The optimal conditions for heterotrophic nitrification were determined as follows: sodium succinate as carbon source, culture temperature 30-35℃, initial pH 8.0-9.0, rotation speed 150-200r/min. Under the optimal culture conditions for 9h, 90.64 ％ of ammonia nitrogen with initial concentration of 107.52 mg/L could be removed there was no accumulation of nitrite nitrogen and the nitrate nitrogen content was always lower than 3.5mg/L in the whole process. The removal rate of total nitrogen is up to 88.63％. The experimental results showed that the strain WUST-7 had good simultaneous nitrification and denitrification potential when it used ammonia nitrogen for nitrification and nitrate nitrogen for denitrification.关键词
废水/异养硝化/好氧反硝化/低C/N比/同步硝化反硝化Key words
waste water/heterotrophic nitrification/aerobic denitrification/low C/N ratio/simultaneous nitrification and denitrification分类
资源环境引用本文复制引用
胡杰,颜家保,霍晓琼,陈美玲,李超..低C/N比异养硝化-好氧反硝化菌筛选及硝化特性[J].化工进展,2019,38(3):1567-1572,6.基金项目
湖北省科技创新专项重大项目(2017ACA179). （2017ACA179）
