黑龙江农业科学Issue(2)：6-9,4.DOI:10.11942/j.issn1002-2767.2019.02.0006
基于SSR分子标记的萝卜遗传多样性研究
Study on Genetic Diversity of Radish Based on SSR Molecular Markers
李世升 1张娜娜 1陈亚珠 1李竟才 1方元平 1项俊1
作者信息
- 1. 大别山特色资源开发湖北省协同创新中心, 湖北省经济林木种质改良重点实验室, 黄冈师范学院 生命科学学院, 湖北 黄州 438000
- 折叠
摘要
Abstract
In order to promote the preservation and breed improvement of radish germplasm resources, nine varieties of radish were used as the experimental materials, which tried to study on genetic diversity the local and conventional radish varieties mentioned above using SSR.Firstly, field characters (leaf shape and skin color) of the above radish materials were observed, then, 9 pairs of SSR primers were screened to detect the above radish samples.The genetic diversity of nine radish species was analyzed by electrophoretic banding and NTSYS software.The results showed that the nine varieties of radish were divided into two groups, group 1 includes 8 varieties except Qiyehong radish, and each variety showed diversity among different similarity coefficients, group 2 includes only Qiyehong radish.There were two distinct branches in the first group, one branch includes four radishes, Lixiangdagen radish, 734 radish, General radish, and Huangzhou radish, whose skin color is white;another branch includes four radishes, the Yichihong radish, Hongbao radish, 262 radish, and Red garden radish, whose skin color is red, which was consistent with field observations.关键词
聚丙烯酰胺凝胶电泳/SSR分子标记/萝卜/遗传多样性/聚类分析Key words
polyacrylamide gel electrophoresi/SSR molecular markers/radish/genetic diversity/clustering a-nalysis引用本文复制引用
李世升,张娜娜,陈亚珠,李竟才,方元平,项俊..基于SSR分子标记的萝卜遗传多样性研究[J].黑龙江农业科学,2019,(2):6-9,4.
