黑龙江农业科学Issue(2)：28-36,9.DOI:10.11942/j.issn1002-2767.2019.02.0028
枯草芽孢杆菌HAB-8菌株分离筛选鉴定及抑菌机理初步研究
Isolation, Screening, Identification and Antimicrobial Mechanism of Bacillus amyloliquefaciens Strain HAB-8
摘要
Abstract
This research was on the purpose to study the biological characteristics and antimicrobial mechanism of Bacillus subtilis strain HAB-8.First, morphology, physiological and biochemical characteristics and 16 S rDNA gene sequence were used for identification of HAB-8 strains; second, the antibacterial activity was detected by flat confrontation experiments.At the same time, the adaptability of different nutritional conditions, salt tolerance was determination; Last, we used optical microscopy and scanning electron microscopy to explore its inhibitory mechanism.The results showed that the identification of bacterial strains HAB-8 was B.subtilis by morphological, physiological and biochemical characteristics, and phylogenetic analysis of 16 srDNA; The colony morphology of HAB-7 is obviously different in different medium plate, colony diameter ranged from 16.25-22.50 mm;HAB-8 available in 0-20％ NaCl growth conditions; HAB-8 confrontation of 18 species of pathogenic fungi, and its average bacteriostatic rate in 58.52％; there were different morphological changes in the mycelia of the treatment group, and the mycelium was distorted, the central part of mycelia was enlarged, and the spore was deformed; under scanning electron microscopy, we found the bacteria directly contact with mycelium and cause deformities of mycelium and spores.It is suggested that antagonistic bacteria may inhibit the growth of plant pathogenic fungi not only by secreting active substances, but also by direct action.关键词
Bacillus subtilis/HAB-8/抑菌活性/拮抗机理/扫描电镜Key words
Bacillus subtilis/HAB-8 strain/morphological observation/antifungal activity/mechanism of action引用本文复制引用
李琪敏,周婷婷,秦春秀,何其光,缪卫国,刘文波..枯草芽孢杆菌HAB-8菌株分离筛选鉴定及抑菌机理初步研究[J].黑龙江农业科学,2019,(2):28-36,9.基金项目
海南大学青年教师基金(hdkyxj201708) （hdkyxj201708）
海南省自然科学基金(20163040,20153131) （20163040,20153131）
南繁区生物安全监测预警及控制关键技术研究与示范(201403075) （201403075）
海南省产学研项目(CXY20140038) （CXY20140038）
