河南农业科学2018，Vol.47Issue(12)：64-69,6.DOI:10.15933/j.cnki.1004-3268.2018.12.010
基于高通量测序的生物炭施用量对植烟土壤细菌群落的影响
Effects of Application Amount of Biochar on Soil Bacterial Community in Tobacco Fields Based on High-throughput Sequencing
摘要
Abstract
High-throughput sequencing of root zoon soil of tobacco K326 of treatments with original soil(OEE), no biochar and biochar of 0.75, 1.5, 2.25 t/ha were done, and the diversity and composition of bacteria were analyzed, so as to provide reference for improvement of mountain soils by biochar.The results showed that, compared with CK, Chao1, Shannon indices of treatments with biochar decreased, the community abundances of Proteobacteria, Chloroflexi, Armatimonadetes increased, the community abundance of Acidobacteria decreased, the community abundance of Saccharibacteria, Gemmatimonadetes and Actinobacteria changed with the application rates of biochar.There was higher similarity for dominant phyla composition among treatments with biochar, but lower between OEE and treatments with biochar, the similarity for dominant phyla composition between CK and treatments with biochar was higher than that between OEE and treatments with biochar.关键词
生物炭/植烟土壤/微生物/多样性/高通量测序Key words
Biochar/Tobacco-growing soil/Microorganism/Diversity/High-throughput sequencing分类
农业科技引用本文复制引用
任天宝,杨艳东,高卫锴,李宙文,阎海涛,王省伟,刘国顺..基于高通量测序的生物炭施用量对植烟土壤细菌群落的影响[J].河南农业科学,2018,47(12):64-69,6.基金项目
国家重点研发计划项目(2017YFD0200808) （2017YFD0200808）
基于"双喜"品牌原料需求的湘西山地特色优质烟叶开发关键技术研究[粤烟15XM-QK(2013)-01] （2013）
登封地区植烟土壤生态保育及修复技术研究及利用(ZYKJ201501) （ZYKJ201501）
