河南农业科学2018，Vol.47Issue(12)：96-101,6.DOI:10.15933/j.cnki.1004-3268.2018.12.015
百合鳞茎腐烂病病原菌的鉴定及生物学特性研究
Identification and Biological Characteristics of the Pathogen of Lily Bulb Rot Disease
摘要
Abstract
To determine the pathogen causing lily bulb rot disease in Changping district, Beijing city, the pathogens were identified by tissue isolation, pathogenicity test, morphological characteristics study and r DNA-ITS sequence analysis.Biological characteristics of the major pathogen were determined by measuring the diameter of the colony and counting the number of conidia using hemocytometer measurement.The results showed that Fusarium oxysporum and Fusarium solani were identified as the pathogens causing bulb rot in lily.F.oxysporum was the dominant strain although its pathogenicity was weaker than F.solani.For the mycelial growth and sporulation of F.oxysporum, the optimum medium was PDA, the optimum temperature was 25℃, and the optimum light condition was 12∶12 alternation of light and darkness.The optimal pH values for mycelial growth and sporulation were 5 and 7, respectively.Lactose was the best carbon source and NaNO3was the best nitrogen source for mycelial growth.The optimum carbon source and nitrogen source for sporulation were glucose and glycine separately.关键词
百合/鳞茎腐烂病/病原菌鉴定/尖孢镰刀菌/生物学特性Key words
Lily/Bulb rot disease/Pathogen identification/Fusarium oxysporum/Biological characteristics分类
农业科技引用本文复制引用
曹兴,刘南南,胡燕佩,义鸣放,王桂清..百合鳞茎腐烂病病原菌的鉴定及生物学特性研究[J].河南农业科学,2018,47(12):96-101,6.基金项目
国家自然科学基金项目(31601788) （31601788）
山东省自然科学基金项目(ZR2016CB36) （ZR2016CB36）
聊城大学博士科研启动基金项目(318051312) （318051312）
国家级大学生创新创业训练计划项目(201710447107,201810447074) （201710447107,201810447074）
