摘要
Abstract
Objective To study the effect of 7, 8-dihydroxyflavone (7, 8-DHF) and Schwann cells (SCs) transplantation on axon regeneration and function recovery after acellular nerve allograft (ANA) repair of the sciatic nerve gap in mice.Methods A total of 45 healthy SD rat were randomly divided into control group, SCs group and 7, 8-DHF + SCs group. SFI and electrophysiology were used to evaluate functional recovery. BDNF and TrkB protein were evaluated by western bolt, observe the fluorescence signal of SCs labeled with PKH-26 by using immunofluorescence, observe the axonal regeneration and myelination by using NF, S100 immunofluorescence. Results Compared with SCs group, the expression of BDNF, TrkB, NF, S100 and PKH-26 labeled SCs in 7, 8-DHF + SCs group were increased (P＜ 0. 05). Compared with control group, nerve conduction velocity and wave amplitude were significantly increased in SCs group and 7, 8-DHF + SCs group (P ＜ 0. 05), however, incubation period were significantly decreased in two treatment group (P ＜ 0. 05), the effect of 7, 8-DHF + SCs group was more powerful than SCs group (P＜ 0. 05), moreover, the SFI score of 7, 8-DHF + SCs group was higher than control group a nd SCs group. Conclusion The combined 7, 8-DHF and SCs transplantation treatment promoted axonal regeneration, myelination and function recovery significantly more than SCs treatments, which may be related to 7, 8-DHF upregulating BDNF and TrkB expression in the ANA, and promoting SCs survival.关键词
7,8-二羟基黄酮/施万细胞/坐骨神经损伤/脑源性神经营养因子/周围神经缺损Key words
7, 8-dihydroxyflavone/Schwann cells/Acellular nerve allograf/BDNF/Peripheral nerve defect分类
医药卫生
