河南中医2019，Vol.39Issue(4)：531-534,4.DOI:10.16367/j.issn.1003-5028.2019.04.0133
祛胰抵方对胰岛素抵抗3T3-L1脂肪细胞脂联素、瘦素及抵抗素表达的影响
The Influence of Formula for Removing Insulin on the Expression of 3T3-L1 Adipocyte Adiponectin and Leptin and Resistin
摘要
Abstract
Objective:To observe the influence of Formula for Removing Insulin on the expression of 3 T3-L1 adipocyte adiponectin and leptin and resistin, and clarify its mechanism in treating insulin resistance of type 2 diabetes mellitus (T2 DM).Methods:The adipocytes before 3 T3-L1 were induced to differentiate into mature adipocytes by cell culture technique.IR cell model was established by co-induction of dexamethasone and insulin.The adipocytes were divided into the control group, the model group, the pioglitazone group, the high-dose FRIR group, the medium-dose FRIR group and the low-dose FRIR group.Glucose consumption in culture medium was detected by glucose oxidase method, and the secretion levels of adiponectin, leptin and resistin were detected by enzyme-linked immunosorbent assay (ELISA).Results:Compared with the control group, the content of adiponectin in the model group decreased, while the content of leptin and resistin increased.And all the differences were statistically significant (P<0.05).Compared with the model group, the contents of adiponectin of the high-dose FRIR group, the medium-dose FRIR group the low-dose FRIR group, and the pioglitazone group increased significantly, while the contents of leptin and resistin decreased significantly, and all the differences were statistically significant (P<0.05).The content of adiponectin of the medium-dose FRIR group was higher than that of the high-dose FRIR group and that of the low-dose FRIR group.The resistin and leptin of the high-dose FRIR group were lower than those of the medium-dose FRIR group and those of the low-dose FRIR group, and all the differences were statistically significant (P<0.05).Conclusion:Formula for Removing Insulin Resistance can increase the secretion of adiponectin and reduce the secretion of leptin and resistin, thus improving IR.关键词
胰岛素抵抗/祛胰抵方/3T3-L1脂肪细胞/脂联素/抵抗素/瘦素Key words
insulin resistance (IR)/Formula for Removing Insulin Resistance/3T3-L1 adipocytes/adiponectin/resistin/leptin分类
医药卫生引用本文复制引用
牛晓瑞,马建,赵娜..祛胰抵方对胰岛素抵抗3T3-L1脂肪细胞脂联素、瘦素及抵抗素表达的影响[J].河南中医,2019,39(4):531-534,4.基金项目
黑龙江中医药大学科研基金项目(201411) （201411）
