吉林农业大学学报2018，Vol.40Issue(6)：687-693,7.DOI:10.13327/j.jjlau.2018.3634
植物生长物质及培养基对红豆越橘叶片离体再生的影响
Effects of Plant Growth Substances and Culture Media on in Vitro Regeneration of Lingonberry Leaves
摘要
Abstract
The effects of five kinds of plant growth substances and four culture media on regenerating adventitious bud from plantlet leaves in vitro were studied to provide the basis for the establishment of high efficient regeneration system with lingonberry( Vaccinium vitis-idaea L.) cultivar"Ida". The results showed that higher adventitious bud induction rate and greater bud number were obtained by using ZT, TDZ or CPPU alone, in which the better treatments are: 88. 3％ induction rate and 21. 3 buds per leaf with CPPU 3 mg/L, respectively, 87. 5％ and 23. 2 bud/leaf with TDZ 1. 0 mg/L, 91. 7％ and 23. 6 bud/leaf with ZT 4. 0 mg/L. TDZ or CPPU inhibited the growth of adventitious buds. ZT 4. 0 mg/L+IBA 0. 1 mg/L was the ideal combination, of which the induction rate and the number of adventitious buds were 82. 5％ and 33. 2 bud/leaf, respectively. The elongation of adventitious buds was promoted at low concentration of IBA or NAA, but inhibited at high concentration. The effect of adventitious bud induction and growth in WPM media is better than that in MS, 1/2 MS & 1/4 MS.关键词
红豆越橘/组织培养/植物生长物质/不定芽再生Key words
lingonberry/tissue culture/plant growth substance/adventitious bud regeneration分类
农业科技引用本文复制引用
王静,张志东,孙海悦,刘海广,唐雪东,李亚东..植物生长物质及培养基对红豆越橘叶片离体再生的影响[J].吉林农业大学学报,2018,40(6):687-693,7.基金项目
吉林省科技发展计划项目(20150414047GH, 20160203009NY) （20150414047GH, 20160203009NY）
吉林省省级经济结构战略调整引导资金专项(2015Y060) （2015Y060）
长春市科技计划项目(2014182) （2014182）
