吉林农业大学学报2018，Vol.40Issue(6)：700-706,7.DOI:10.13327/j.jjlau.2018.4528
响应面法优化救必应提取纯化工艺及其治疗肝炎的研究
Optimization of Extraction and Purification Process of Ovateleaf Holly Bark by Response Surface Methodology and Its Hepatoprotective Effects on CCl4-induced Liver Injury
摘要
Abstract
The ethyl acetate-butyl acetate extraction and polyamide resin purification of ovateleaf holly bark were investigated in this work. The independently investigated parameters of the extraction process included extraction time, solid-to-liquid ratio, mixing ratio of extract, mixing ratio of polyamide and crude drug, polyamide ratio of mixed medicine and non-mixed medicine, and eluent concentration. The optimized extraction conditions were determined with the aid of response surface analysis. Results indicated that the extraction yield of ovateleaf holly bark was up to 95. 41％ under the optimized conditions: extraction time 124 min, solid-to-liquid ratio at 1 ∶ 21( g ∶ mL), mixingratio of extract at 1 ∶ 2, mixing ratio of polyamide and crude drug at 3 ∶ 1, polyamide ratio of mixed medicine and non mixed medicine at 1 ∶ 3. To determine the liver protection and anti-inflammation of ovateleaf holly bark extract, we used the established acute liver injury mouse mode. Results showed that significant inhibitory effect on liver injury was observed, and the inhibition rate was up to 86. 09％. The changes in tissue sections also indicated protective effect of liver. Results also showed a significant reduction of ALT and AST activity( P ＜ 0. 01) and the formation of MDA and the expression of COX-2 were significantly suppressed. These data suggest that the ovateleaf holly bark extract has the functions of liver protection and anti-inflammation, and good prospects for development and application.关键词
救必应提取物/聚酰胺树脂/得率/保肝/抗炎Key words
ovateleaf holly bark extract/polyamide resin/yield/liver protection/anti-inflammation分类
医药卫生引用本文复制引用
赵立春,廖夏云,唐梅,扈芷怡,张谦华,庞宇舟..响应面法优化救必应提取纯化工艺及其治疗肝炎的研究[J].吉林农业大学学报,2018,40(6):700-706,7.基金项目
广西科技攻关计划项目(0201205-1-5) （0201205-1-5）
广西中医药大学"岐黄工程"高层次人才团队培育项目(2018002) （2018002）
