吉林农业大学学报2019，Vol.41Issue(1)：42-47,6.DOI:10.13327/j.jjlau.2018.3432
注射用大黄素脂质体的制备及质量评价
Preparation and Quality Evaluation of Emodin Liposomes for Injection
摘要
Abstract
To establish the entrapment efficiency determination of emodin liposomes, emodin liposome for injection with high entrapment efficiency was prepared and its quality was evaluated. Non encapsulated drugs were expected by centrifugal settling-micromembrane extruding method. The entrapment efficiency was determined by HPLC. The preparation of emodin liposome for injection was conducted by thin film dispersion-freeze drying method. The entrapment efficiency was taken as the evaluation index, and the preparation technology was established. The entrapment efficiency, the surface morphology, the mean particle diameter, the zeta potential and the storage stability were studied. The optimized preparation conditions were as follows: the ratio of lecithin-cholesterol-emodin was 70:2:3, double-distilled water was hydrated fluid, pre-freezing temperature was 10 ℃, sucrose was the protective agent, the ratio of sucrose-water was 1:10, and 5％ glucose injection was rehydrated fluid. The entrapment efficiency was above 80％, the surface morphology was regular sphere, the mean particle diameter was (206.3±17.5) nm, the zeta potential was (-31.6±0.8) mV and the storage time was longer than 3 months in 4 ℃ sealed environment.关键词
注射用大黄素脂质体/薄膜分散-冷冻干燥法/包封率Key words
emodin liposome for injection/thin film dispersion-freeze drying method/entrapment efficiency分类
医药卫生引用本文复制引用
刘昊,焦连庆,于敏,焦安妮,张晶..注射用大黄素脂质体的制备及质量评价[J].吉林农业大学学报,2019,41(1):42-47,6.基金项目
吉林省科技发展计划项目(20130727026YY) （20130727026YY）
