集美大学学报（自然科学版）2019，Vol.24Issue(1)：14-19,6.DOI:10.19715/j.jmuzr.2019.01.03
鱼肉中LMG的分子印迹仿生ELISA检测方法
Detection of Leuco Malachite Green in Fish by Biomimetic ELISA Method
摘要
Abstract
For the detection of leuco malachite green (LMG) in aquatic products, a biomimetic ELISA method was established based on the biomimetic antibody membrane of molecularly imprinted polymer (MIP).Using LMG as template molecules, the LMG-MIP membrane could be formed on the surface of microplates through the dopamine (DA) self-polymerization for 4 h in Tris-HCl (p H = 8. 5). After elution of LMG by methanol-acetic acid at the volume ratio of 9∶ 1, the LMG-MIP membrane on microplates could be used as biomimetic antibody of LMG in ELISA. The sensitivity estimated with IC50 was 5. 18 μg/L and the detection limit was0. 03 μg/L. The cross-reactivities for two structural analogues of LMG were 21. 3％ for malachite green. The recovery tested by LMG standard spiking in fish samples ranged from 71. 8％ to 73. 5％, and the average relative standard deviation was less than 4. 2％. The results showed that the method could be used for the rapid detection of LMG residues in fish.关键词
隐性孔雀石绿/酶联免疫吸附法/分子印迹仿生抗体/鱼肉Key words
leuco malachite green/ELISA/molecularly imprinted biomimetic antibody/fish分类
农业科技引用本文复制引用
彭爱红,王茜雅,林郑忠,陈晓梅,黄志勇..鱼肉中LMG的分子印迹仿生ELISA检测方法[J].集美大学学报（自然科学版）,2019,24(1):14-19,6.基金项目
福建省自然科学基金项目 (2015J01615) （2015J01615）
福建省科技计划项目 (2016Y0064) （2016Y0064）
福建省海洋功能食品工程中心开放课题 (C11172) （C11172）
集美大学创新团队基金项目 (2010A007) （2010A007）
