解剖学杂志(社会科学版)2019，Vol.42Issue(2)：111-115,5.DOI:10.3969/j.issn.1001-1633.2019.02.001
小鼠非同步胚胎移植技术
Asynchronous embryo transfer technique in mice
摘要
Abstract
Objective:To investigate the asynchronous embryo transfer in mice.Methods:Preimplantation embryos at different developmental stages were asynchronously transferred into the unilateral oviduct or uterine horn of pseudopregnant recipient mice with different post-coitum days,and the effect of asynchronous embryo transfer on success rate of embryo transfer and newborn birth weight was evaluated.Results:All of the zygotes,2-cell embryos,morula,and blastocysts could be transferred into unilateral oviduct of pseudopregnant 0.5 day recipients and developed to live newborns 19 days after embryo transfer.The birth rates of the transferred zygotes,2-cell embryos,morula,and blastocysts were 56.3%,62.5%,59.4%,and 58.3%,respectively.Morula and blastocysts could still be nonsurgically transferred into unilateral uterine horns of pseudopregnant 2.5 day recipients and developed into live newborns 17 days after embryo transfer.The birth rates of the transferred morula and blastocysts were 57.5% and 62.5%,respectively.The birth weight of newborns from the transferred blastocysts into oviducts of pseudopregnant 0.5 day recipients was significantly increased than that of newborns from the transferred blastocysts into uterine horns of pseudopregnant 2.5 day recipients.Conclusion:If embryos at different developmental stages are transferred into recipients with the same post-coitum days,the pregnant recipients would have the same gestation.If embryos at the same developmental stage are transferred into recipients with different post-coitum days,the pregnant recipients would have different gestation.The pregnant recipients with short post-coitum days have longer gestation and significantly increased birth weight of newborns.关键词
非同步胚胎移植/子宫/输卵管/出生重/小鼠Key words
asynchronous embryo transfer/uterine horn/oviduct/birth weight/mouse引用本文复制引用
高晓茜,陈培勇,张帅,栗金丽,刘倩,李华..小鼠非同步胚胎移植技术[J].解剖学杂志(社会科学版),2019,42(2):111-115,5.基金项目
国家重点研发计划项目(2017YFD0501901) （2017YFD0501901）
河北农业大学大学生创新创业训练计划项目(201810086012) （201810086012）
