解剖学杂志(社会科学版)2019，Vol.42Issue(2)：152-154,160,4.DOI:10.3969/j.issn.1001-1633.2019.02.010
HIL-15基因修饰的NK-92细胞治疗黑色素瘤
Treatment in the melanoma with HIL-15 gene modified NK-92 cells
摘要
Abstract
Objective:To investigate whether NK-92 cells modified with hyper-IL-15(HIL-15)gene which was composed of IL-15 and the domain of the IL-15 receptor chain could promote the proliferation of NK-92 cells and enhance their killing effect on melanoma in mice.Methods:CCK-8 assay was used to detect the proliferation differences between NK-92 cells and HIL-15NK-92 cells.Blank control group,PBS group,NK-92 treatment group and HIL-15NK-92 treatment group were established.Four C57BL/6 male mice in each group were subcutaneously inoculated with B16-F10 melanoma cells.The growth of melanoma and the survival time of mice were observed and recorded.Results:Compared with NK-92 cells,the proliferation activity of NK-92 cells after HIL-15 gene modification was enhanced.The survival time of mice in HIL-15NK-92 treatment group was longer than that in NK-92 treatment group.Conclusion:HIL-15 gene modification can promote the proliferation of NK-92 cells and prolong the survival time of melanoma bearing mice.关键词
NK-92细胞/基因修饰/细胞增殖/免疫治疗Key words
NK-92 cell line/gene modification/cell proliferation/immune therapy引用本文复制引用
于淮海,孙雨飞,刘祥,金昌洙,付强..HIL-15基因修饰的NK-92细胞治疗黑色素瘤[J].解剖学杂志(社会科学版),2019,42(2):152-154,160,4.基金项目
国家自然科学基金(81370730,81571512) （81370730,81571512）
