实用临床医药杂志2019，Vol.23Issue(8)：1-6,6.DOI:10.7619/jcmp.201908001
薏苡仁油诱导胃癌细胞SGC-7901凋亡的实验研究
Experimental study on apoptosis of gastric cancer cell line SGC-7901 induced by coix seed oil
摘要
Abstract
Objective To investigate the possible mechanism of coix seed in inducing apoptosis of gastric cancer cells (SGC-7901). Methods Different concentrations of coix seed oil (2, 4, 8 mg/mL) were applied to SGC-7901 cells. MTT assay was used to detect the effect of drugs on cell proliferation, and flow cytometry for drug-induced cell apoptosis, scratch test for cell migration inhibited by coix seed oil, Transwell chamber for drug-inhibited cell invasion, and Western blot for expression of related proteins PRMT5, PI3 K and AKT. Results The results of MTT showed that 2, 4 mg/mL of coix seed oil could significantly inhibit the proliferation of SGC-7901 cells, and the inhibition rate of 2 mg/mL was (30. 02 ± 1. 56) ％, which showed significant difference compared to the control group (P ＜ 0. 01). The results of flow cytometry showed that the apoptosis rates of coix seed oil cells at concentrations of 2 and 4 mg/mL were (16. 25 ± 2. 54) ％, (12. 60 ± 1. 12) ％, respectively, and which showed a significant difference compared with (2. 0 ± 1. 22) ％ in the control group (P ＜ 0. 01). The results of cell scratch test showed that the migration of SGC-7901 cells treated with 2, 4 mg/mL of coix seed oil had significant difference when compared to control group (P ＜0. 01). The results of invasion experiments showed that 2, 4 mg/mL of coix seed oil could significantly inhibit cell invasion, and the number of cell invasion was (134. 00 ± 2. 86), (167. 00 ±0. 99), respectively, which showed significant difference compared to (268. 00 ± 2. 05) in the control group (P ＜ 0. 01). The migration number in 8 mg/mL coix seed oil group was (167 ± 0. 99), a significant difference was observed when compared to the control group (P ＜ 0. 05). Western blot analysis showed that different concentrations of coix seed oil could significantly down-regulate the expression of PRMT5, PI3 K and AKT in SGC-7901 cells. Conclusion Coix seed oil can significantly inhibit the proliferation, migration and invasion of gastric cancer SGC-7901 cells, its possible mechanism is to down-regulate the signal pathway of PRMT5-PI3 K/AKT to inhibit the activation of various anti-apoptotic molecules, induce apoptosis and suppress invasion and metastasis of gastric cancer SGC-7901 cells by down-regulating the PRMT5-PI3 K/AKT signaling pathway.关键词
薏苡仁油/胃癌/蛋白质精氨酸甲基转移酶5/迁移Key words
coix seed oil/gastric cancer/protein arginine methyltransferase 5/migration分类
医药卫生引用本文复制引用
孙国壮,曹有军,毛海燕,罗恒,何子渡,孔桂美..薏苡仁油诱导胃癌细胞SGC-7901凋亡的实验研究[J].实用临床医药杂志,2019,23(8):1-6,6.基金项目
国家自然科学基金资助项目(81272537) （81272537）
国家临床重点专科项目(SRD20180141) （SRD20180141）
扬州大学大学生学术科技创新基金项目(X20170885,X20160782,2016CXJ094) （X20170885,X20160782,2016CXJ094）
