检验医学与临床2019，Vol.16Issue(3)：289-291,3.DOI:10.3969/j.issn.1672-9455.2019.03.001
不同离心力分离外周血单个核细胞的条件优化
Condition optimization of different centrifugal force on separating peripheral blood mononuclear cells
摘要
Abstract
Objective To observe the effect of different centrifugal force on the separation of peripheral blood mononuclear cell (PBMC) .Methods PBMCs were isolated by adopting the ficoll density gradient centrifugation at different centrifugal force of separation and centrifugal force of washing.Then the yield rate and survival status of PBMCs were measured by flow cytometry and the statistical analysis was performed.Results The results of separation centrifugal force:the maximum number of PBMCs was obtained at 600 g.The number of apoptotic cells between 300-900 g separation in detection at 24 hhad no statistically significant difference, moreover was relatively less, increasing centrifugal force, the number of apoptosis cells was increased;in the detection at 72 h, the centrifugal force higher than 1 200 g or low to 300 g, the survival cells were significantly decreased.The results of washing centrifugal force:the centrifugal force was 150 g, the yield rate of PBMCs was significantly lower than that of 300-2 400 g.The apoptotic cells detected at 24 hshowed that the centrifugal force was more than 600 g, the number of apoptosis cells was increased;the detection at 72 h showed that the washing centrifugal force was more than 1 200 g, and the number of survival cells was decreased significantly.Conclusion The centrifugal force of separation is 600-900 g and centrifugal force of washing is 300-600 g, which all can be used for the separation of PBMC.关键词
外周血单个核细胞/聚蔗糖密度梯度离心法/离心力Key words
peripheral blood mononuclear cell/ficoll density gradient centrifugation/centrifugal force分类
医药卫生引用本文复制引用
熊静,王思雨,张云惠,吴利红,邓弘仙,刘阳,罗兴燕..不同离心力分离外周血单个核细胞的条件优化[J].检验医学与临床,2019,16(3):289-291,3.基金项目
国家自然科学基金资助项目(81302786) （81302786）
成都医学院自然基金项目(CYTD15-01 （）
CYZ15-13) （）
大学生国家创新项目(201513705048). （201513705048）
